Comparative genomic analysis of uropathogenic Escherichia coli strains from women with recurrent urinary tract infection

Front Microbiol. 2024 Jan 24:14:1340427. doi: 10.3389/fmicb.2023.1340427. eCollection 2023.

Abstract

Introduction: Recurrent urinary tract infections (RUTIs) caused by uropathogenic Escherichia coli are costly public health problems impacting patients' quality of life.

Aim: In this work, a comparative genomics analysis of three clinical RUTI strains isolated from bladder biopsy specimens was performed.

Materials and methods: One hundred seventy-two whole genomes of urinary tract E. coli strains were selected from the NCBI database. The search for virulence factors, fitness genes, regions of interest, and genetic elements associated with resistance was manually carried out. The phenotypic characterization of antibiotic resistance, haemolysis, motility, and biofilm formation was performed. Moreover, adherence and invasion assays with human bladder HTB-5 cells, and transmission electron microscopy (TEM) were performed.

Results: The UTI-1_774U and UTI-3_455U/ST1193 strains were associated with the extraintestinal pathotypes, and the UTI-2_245U/ST295 strain was associated with the intestinal pathotype, according to a phylogenetic analysis of 172 E. coli urinary strains. The three RUTI strains were of clinical, epidemiological, and zoonotic relevance. Several resistance genes were found within the plasmids of these strains, and a multidrug resistance phenotype was revealed. Other virulence genes associated with CFT073 were not identified in the three RUTI strains (genes for type 1 and P fimbriae, haemolysin hlyA, and sat toxin). Quantitative adherence analysis showed that UTI-1_774U was significantly (p < 0.0001) more adherent to human bladder HTB-5 cells. Quantitative invasion analysis showed that UTI-2_245U was significantly more invasive than the control strains. No haemolysis or biofilm activity was detected in the three RUTI strains. The TEM micrographs showed the presence of short and thin fimbriae only in the UTI-2_245U strain.

Conclusion: The high variability and genetic diversity of the RUTI strains indicate that are a mosaic of virulence, resistance, and fitness genes that could promote recurrence in susceptible patients.

Keywords: MDR; RUTIs; UPEC; WGS; adherence.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. The study described in this manuscript was supported by Public Federal Funds grant numbers HIM/2018/072 SSA. 1527, HIM/2021/030 SSA. 1730, HIM/2021/031 SSA. 1720, and HIM/2022/014 SSA. 1777 from the HIMFG. This work was also supported by the “Consejo Nacional de Humanidades, Ciencias y Tecnologías (CONAHCYT)” with number grant 319563 at the “Announcement for Basic Science and/or Frontier Science, Modality: Paradigms and Controversies of Science 2022”.