The InBIO barcoding initiative database: DNA barcodes of Iberian Trichoptera, documenting biodiversity for freshwater biomonitoring in a Mediterranean hotspot

Joana Pauperio; Luis Martin Gonzalez; Jesus Martinez; Marcos A González; Filipa Ms Martins; Joana Veríssimo; Pamela Puppo; Joana Pinto; Cátia Chaves; Catarina J Pinho; José Manuel Grosso-Silva; Lorenzo Quaglietta; Teresa Luísa L Silva; Pedro Sousa; Paulo Celio Alves; Nuno Fonseca; Pedro Beja; Sónia Ferreira

doi:10.3897/BDJ.11.e97484

The InBIO barcoding initiative database: DNA barcodes of Iberian Trichoptera, documenting biodiversity for freshwater biomonitoring in a Mediterranean hotspot

Biodivers Data J. 2023 Jan 19:11:e97484. doi: 10.3897/BDJ.11.e97484. eCollection 2023.

Authors

Joana Pauperio^{1

2

3}, Luis Martin Gonzalez⁴, Jesus Martinez⁴, Marcos A González⁴, Filipa Ms Martins^{3

1}, Joana Veríssimo^{3

1

5}, Pamela Puppo⁶, Joana Pinto^{3

1}, Cátia Chaves^{3

1}, Catarina J Pinho^{3

1

5}, José Manuel Grosso-Silva⁷, Lorenzo Quaglietta^{8

1}, Teresa Luísa L Silva^{3

1}, Pedro Sousa^{3

1}, Paulo Celio Alves^{3

1

9}, Nuno Fonseca^{3

1}, Pedro Beja^{1

8

3}, Sónia Ferreira^{3

1

9}

Affiliations

¹ BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO, Campus de Vairão, 4485-661 Vairão, Vila do Conde, Portugal BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO, Campus de Vairão, 4485-661 Vairão Vila do Conde Portugal.
² European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, United Kingdom European Molecular Biology Laboratory, European Bioinformatics Institute Hinxton, Cambridge United Kingdom.
³ CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Campus de Vairão, Universidade do Porto, 4485-661 Vairão, Vila do Conde, Portugal CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Campus de Vairão, Universidade do Porto, 4485-661 Vairão Vila do Conde Portugal.
⁴ Departamento de Zoología, Genética y Antropología Física, Facultad de Biología. Universidad de Santiago de Compostela, Santiago de Compostela, Spain Departamento de Zoología, Genética y Antropología Física, Facultad de Biología. Universidad de Santiago de Compostela Santiago de Compostela Spain.
⁵ Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, 4169-007, Porto, Portugal Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, 4169-007 Porto Portugal.
⁶ Marshall University, Department of Biological Sciences, Huntington, United States of America Marshall University, Department of Biological Sciences Huntington United States of America.
⁷ Museu de História Natural e da Ciência da Universidade do Porto, Porto, Portugal Museu de História Natural e da Ciência da Universidade do Porto Porto Portugal.
⁸ CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Instituto Superior de Agronomia, Universidade de Lisboa, Lisboa, Portugal CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Instituto Superior de Agronomia, Universidade de Lisboa Lisboa Portugal.
⁹ EBM, Estação Biológica de Mértola, Praça Luís de Camões, Mértola, Portugal EBM, Estação Biológica de Mértola, Praça Luís de Camões Mértola Portugal.

Abstract

Background: The Trichoptera are an important component of freshwater ecosystems. In the Iberian Peninsula, 380 taxa of caddisﬂies are known, with nearly 1/3 of the total species being endemic in the region. A reference collection of morphologically identified Trichoptera specimens, representing 142 Iberian taxa, was constructed. The InBIO Barcoding Initiative (IBI) Trichoptera 01 dataset contains records of 438 sequenced specimens. The species of this dataset correspond to about 37% of Iberian Trichoptera species diversity. Specimens were collected between 1975 and 2018 and are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources, Portugal) or in the collection Marcos A. González at the University of Santiago de Compostela (Spain).

New information: Twenty-nine species, from nine different families, were new additions to the Barcode of Life Data System (BOLD). A success identification rate of over 80% was achieved when comparing morphological identifications and DNA barcodes for the species analysed. This encouraging step advances incorporation of informed Environmental DNA tools in biomonitoring schemes, given the shortcomings of morphological identifications of larvae and adult Caddisﬂies in such studies. DNA barcoding was not successful in identifying species in six Trichoptera genera: Hydropsyche (Hydropsychidae), Athripsodes (Leptoceridae), Wormaldia (Philopotamidae), Polycentropus (Polycentropodidae) Rhyacophila (Rhyacophilidae) and Sericostoma (Sericostomatidae). The high levels of intraspecific genetic variability found, combined with a lack of a barcode gap and a challenging morphological identification, rendered these species as needing additional studies to resolve their taxonomy.

Keywords: DNA barcode; Trichoptera; continental Portugal; continental Spain; cytochrome c oxidase subunit I (COI); occurrence records; species distributions.

Joana Pauperio, Luis Martin Gonzalez, Jesus Martinez, Marcos A González, Filipa MS Martins, Joana Veríssimo, Pamela Puppo, Joana Pinto, Cátia Chaves, Catarina J. Pinho, José Manuel Grosso-Silva, Lorenzo Quaglietta, Teresa Luísa L Silva, Pedro Sousa, Paulo Celio Alves, Nuno Fonseca, Pedro Beja, Sónia Ferreira.