Biodistribution and Tumor Targeted Accumulation of Anti-CEA-loaded Iron Nanoparticles

Thais Silva Correa; William Gustavo Lima; Aline Beatriz do Couto Campos; Alexsandro Sobreira Galdino; Emilia Celma de Oliveira Lima; Valbert Nascimento Cardoso; Simone Odília Antunes Fernandes; Mariana Campos-da-Paz

doi:10.2174/0113892010268872240104114444

Biodistribution and Tumor Targeted Accumulation of Anti-CEA-loaded Iron Nanoparticles

Curr Pharm Biotechnol. 2024 Feb 6. doi: 10.2174/0113892010268872240104114444. Online ahead of print.

Authors

Thais Silva Correa¹, William Gustavo Lima², Aline Beatriz do Couto Campos², Alexsandro Sobreira Galdino³, Emilia Celma de Oliveira Lima⁴, Valbert Nascimento Cardoso², Simone Odília Antunes Fernandes², Mariana Campos-da-Paz¹

Affiliations

¹ Department of Biochemistry, Federal University of São João del Rei, Divinópolis, MG, 35500-291, Brazil.
² School of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
³ Laboratory of Microbial Biotechnology, Federal University of Sao Joao Del-Rei, MG, 35500-291, Brazil.
⁴ Institute of Chemistry, Federal University of Goiás, Goiânia, GO Brazil.

PMID: 38321899
DOI: 10.2174/0113892010268872240104114444

Abstract

Introduction: Active targeting of tumors by nanomaterials favors early diagnosis and the reduction of harsh side effects of chemotherapeuticals.

Method: We synthesized magnetic nanoparticles (64 nm; -40 mV) suspended in a magnetic fluid (MF) and decorated them with anti-carcinoembryonic antigen (MFCEA; 144 nm; -39 mV). MF and MFCEA nanoparticles were successfully radiolabeled with technetium-99m (99mTc) and intravenously injected in CEA-positive 4T1 tumor-bearing mice to perform biodistribution studies. Both 99mTc-MF and 99mTc-MFCEA had marked uptake by the liver and spleen, and the renal uptake of 99mTc-MFCEA was higher than that observed for 99mTc-MF at 20h. At 1 and 5 hours, the urinary excretion was higher for 99mTc-MF than for 99mTc-MFCEA.

Results: These data suggest that anti-CEA decoration might be responsible for a delay in renal clearance. Regarding the tumor, 99mTc-MFCEA showed tumor uptake nearly two times higher than that observed for 99mTc-MFCEA. Similarly, the target-nontarget ratio was higher with 99mTc-MFCEA when compared to the group that received the 99mTc-MF.

Conclusion: These data validated the ability of active tumor targeting by the as-developed antiCEA loaded nanoparticles and are very promising results for the future development of a nanodevice for the management of breast cancer and other types of CEA-positive tumors.

Keywords: breast cancer; carcinoembryonic antigen; magnetic nanoparticles; nanotechnology; targeting; theranostic.