Photoacoustic Lifetime Imaging of Hypoxia

Qi Shao; Joshua G Punnoose

doi:10.1007/978-1-0716-3633-6_10

Photoacoustic Lifetime Imaging of Hypoxia

Methods Mol Biol. 2024:2755:141-147. doi: 10.1007/978-1-0716-3633-6_10.

Authors

Qi Shao¹, Joshua G Punnoose^{2

3}

Affiliations

¹ Department of Radiology, University of Minnesota, Minneapolis, MN, USA. shaox070@umn.edu.
² Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, USA.
³ Neurology and Neurosurgery, The Johns Hopkins University, Baltimore, MD, USA.

PMID: 38319575
DOI: 10.1007/978-1-0716-3633-6_10

Abstract

We present an approach based on photoacoustic lifetime imaging (PALI) to map the distribution of oxygen partial pressure (pO₂) in tissue. This method utilizes methylene blue, a dye widely used in clinical applications, as an oxygen-sensitive imaging agent. PALI measurement of oxygen relies upon pO₂-dependent excitation lifetime of the dye. The technique maps the excited triplet state of oxygen-sensitive dye, thus reflecting the spatial and temporal distributions of tissue oxygen.

Keywords: Hypoxia; Methylene blue; Oxygen; Photoacoustic; Photoacoustic lifetime imaging.

MeSH terms

Diagnostic Imaging*
Humans
Hypoxia*
Methylene Blue
Oxygen
Spectrum Analysis

Substances

Oxygen
Methylene Blue