Nuclear pore complex proteins are involved in centromere distribution

Nanami Ito; Takuya Sakamoto; Yuka Oko; Hikaru Sato; Shigeru Hanamata; Yuki Sakamoto; Sachihiro Matsunaga

doi:10.1016/j.isci.2024.108855

Nuclear pore complex proteins are involved in centromere distribution

iScience. 2024 Jan 11;27(2):108855. doi: 10.1016/j.isci.2024.108855. eCollection 2024 Feb 16.

Authors

Nanami Ito¹, Takuya Sakamoto^{2

3}, Yuka Oko³, Hikaru Sato¹, Shigeru Hanamata², Yuki Sakamoto⁴, Sachihiro Matsunaga¹

Affiliations

¹ Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan.
² Department of Science, Faculty of Science, Kanagawa University, Yokohama, Kanagawa 221-8686, Japan.
³ Faculty of Science and Technology, Department of Applied Biological Science, Tokyo University of Science, Noda, Chiba 278-8510, Japan.
⁴ Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.

Abstract

The subnuclear distribution of centromeres is cooperatively regulated by condensin II and the linker of nucleoskeleton and cytoskeleton (LINC) complex. However, other nuclear membrane structures and nuclear proteins are probably involved in centromere dynamics and distribution. Here, we focused on the nuclear pore complex (NPC), which is known to regulate gene expression, transcription memory, and chromatin structure in addition to transport between the cytoplasm and nucleoplasm. We report here that some nucleoporins (Nups), including Nup85, Nup133, CG1, Nup93b, and NUA, are involved in centromere scattering in Arabidopsis thaliana. In addition, the centromere dynamics after metaphase in nup mutants were found to be similar to that of the condensin II mutant. Furthermore, both biochemical and genetic approaches showed that the Nups interact with the LINC complex. These results suggest that Nups regulate centromere scattering cooperatively with condensin II and the LINC complex.

Keywords: Cell biology; Plant biology; Plant cytology.