Effective wound healing on diabetic mice by adhesive antibacterial GNPs-lysine composited hydrogel

Sureerat Khunmanee; Anseo Choi; Il Young Ahn; Woo Ju Kim; Tae Hui Bae; Shin Hyuk Kang; Hansoo Park

doi:10.1016/j.isci.2024.108860

Effective wound healing on diabetic mice by adhesive antibacterial GNPs-lysine composited hydrogel

iScience. 2024 Jan 11;27(2):108860. doi: 10.1016/j.isci.2024.108860. eCollection 2024 Feb 16.

Authors

Sureerat Khunmanee¹, Anseo Choi¹, Il Young Ahn², Woo Ju Kim³, Tae Hui Bae³, Shin Hyuk Kang², Hansoo Park¹

Affiliations

¹ Department of Integrative Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, South Korea.
² Department of Plastic and Reconstructive Surgery, Chung-Ang University Hospital, Chung-Ang University College of Medicine, 102 Heukseok-ro, Dongjak-gu 06973, South Korea.
³ Department of Plastic and Reconstructive Surgery, Chung-Ang University Gwangmyeong Hospital, Deokan-ro, Gwangmyeong-si, Gyeonggi-do 14353, South Korea.

Abstract

Current trends in wound care research focus on creating dressings for diverse wound types, aiming to effectively control the wound healing process. We proposed a wound dressing composed of oxidized hyaluronic acid and amine gelatin with embedded lysine-modified gelatin nanoparticles (HGel-GNPs-lysine). This dressing improves mechanical properties and reduces degradation rates. The storage modulus for HGel-GNPs-lysine was 3,800 Pa, exceeding that of HGel (1,750 Pa). The positively charged surface of GNPs-lysine effectively eliminated Escherichia coli and Staphylococcus aureus. In a diabetic mice model (C57BL/6), HGel-GNPs-lysine immobilized with basic-fibroblast growth factor promoted granulation tissue thickness and collagen density. Gene expression analysis indicated that HGel-GNPs-lysine reduced inflammation and enhanced angiogenesis. This study highlights that HGel-GNPs-lysine could offer alternative treatment strategies for regulating the inflammatory response at the injury site in wound dressing applications.

Keywords: Bioengineering; Biomaterials; Biotechnology; Materials science.