Metabolomics and in-vitro bioactivities studies of fermented Musa paradisiaca pulp: A potential alpha-amylase inhibitor

Adeleke Kazeem Atunnise; Olusola Bodede; Adewale Adewuyi; Vinesh Maharaj; Gerhard Prinsloo; Bamidele Adewale Salau

doi:10.1016/j.heliyon.2024.e24659

Metabolomics and in-vitro bioactivities studies of fermented Musa paradisiaca pulp: A potential alpha-amylase inhibitor

Heliyon. 2024 Jan 19;10(3):e24659. doi: 10.1016/j.heliyon.2024.e24659. eCollection 2024 Feb 15.

Authors

Adeleke Kazeem Atunnise¹, Olusola Bodede^{2

3

4}, Adewale Adewuyi⁵, Vinesh Maharaj², Gerhard Prinsloo³, Bamidele Adewale Salau¹

Affiliations

¹ Department of Biochemistry, Redeemer's University, Ede, Osun state, Nigeria.
² Biodiscovery Center, Department of Chemistry, University of Pretoria, Pretoria, 0028, South Africa.
³ Department of Agriculture and Animal Health, University of South Africa, Florida, 1710, South Africa.
⁴ Antimicrobial Discovery Center, Department of Biology, Northeastern University, Boston, MA, USA.
⁵ Department of Chemical Sciences, Redeemer's University, Ede, Osun state, Nigeria.

Abstract

The in-vitro synthesis of bio-compounds via fermentation is a promising route for bioactive molecules intended for disease control and management. Therefore, this study evaluated the effect of fermentation on the antioxidants, antihyperglycemic and anti-inflammatory properties and the resultant chemometric phytochemical profiles of unripe plantain fruits. The results revealed that Escherichia coli and Propionibacterium spp. are suspected as the key fermenters. The E coli showed negative results to the pathogenicity test; Propionibacterium appeared to be opportunistic. A significant increase in the total polyphenols and protein and decreased flavonoids was recorded in the phytochemical profile of the methanolic extract of the fermented unripe plantain pulp; however, the ascorbic acid content was not significantly altered. The ¹H NMR fingerprint showed that there is a closely related chemical shift among the shorter fermentation time (days 2-6) and the unfermented, while the more extended fermentation periods (days 7-12) with enhanced bioactivities were closely related based on the chemometrics analyses. Furthermore, the UPLC-QTOF-MS analysis annotated the presence of bioactive compounds in the day-9 fermented sample: polyhydroxy glucose conjugates (3-Methoxy-4-hydroxyphenyl 6-O-(3,4,5-trihydroxybenzoyl)-beta-D-glucopyranoside), short chain peptide (leucyl-glycyl-glycine), amino acid derivatives (4-Aminophenylalanine, and N-Acetylhistidine), linear and cyclic fatty acid derivatives (palmitoyl putrescine, ricinoleic acid, phytosphingosine, gabalid, rubrenoic acid, 2-aminocyclopentanecarboxylic and cystodienioc acid). The synergistic effect of these newly formed compounds and the increase in the phenolic content of the day-9 fermented unripe plantain may account for its more potent antioxidant, anti-inflammatory and antihyperglycemic activity. Therefore, the products obtained from the day 9 fermentation of unripe plantain pulp may serve as potential nutraceutical agents against gastro-enteric sugar digestion and absorption and sugar-induced oxidative stress, inflammation and metabolic disease.

Keywords: Antioxidants; Diabetes; Fermentation; Inflammation; Metabolomics; Unripe-plantain pulp.