Chromatin and DNA Dynamics in Mouse Models of Liver Cancers

Abstract

In recent years, important efforts have been made to understand how the expression of a specific gene repertoire correlates with chromatin accessibility, histone mark deposition, as well as with chromatin looping establishing connectivity with regulatory regions. The emergence of new techniques for genome-wide analyses and their progressive optimization to work on low amounts of material allows the scientific community to obtain an integrated view of transcriptional landscapes in physiology and disease. Here, we describe our own experience aiming at correlating the TCF-4/β-catenin cistrome during liver tumorigenesis with chromatin remodeling, histone mark modifications, and long-distance DNA looping.

Keywords: Assay for transposase-accessible chromatin; Chromatin immunoprecipitation; Chromosome conformation capture; Liver tumors; Primary hepatocytes; β-catenin/TCF-4 complex.