Targeted Glycoproteomics Analysis Using MRM/PRM Approaches

Cristian D Gutierrez Reyes; Akeem Sanni; Moyinoluwa Adeniyi; Damir Mogut; Hector R Najera Gonzalez; Parisa Ahmadi; Mojgan Atashi; Sherifdeen Onigbinde; Yehia Mechref

doi:10.1007/978-1-0716-3666-4_14

Targeted Glycoproteomics Analysis Using MRM/PRM Approaches

Methods Mol Biol. 2024:2762:231-250. doi: 10.1007/978-1-0716-3666-4_14.

Authors

Cristian D Gutierrez Reyes¹, Akeem Sanni¹, Moyinoluwa Adeniyi¹, Damir Mogut², Hector R Najera Gonzalez³, Parisa Ahmadi¹, Mojgan Atashi¹, Sherifdeen Onigbinde¹, Yehia Mechref⁴

Affiliations

¹ Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
² Department of Food Biochemistry, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
³ Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX, USA.
⁴ Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA. Yehia.Mechref@ttu.edu.

PMID: 38315369
DOI: 10.1007/978-1-0716-3666-4_14

Abstract

MS-target analyses are frequently utilized to analyze and validate structural changes of biomolecules across diverse fields of study such as proteomics, glycoproteomics, glycomics, lipidomics, and metabolomics. Targeted studies are commonly conducted using multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) techniques. A reliable glycoproteomics analysis in intricate biological matrices is possible with these techniques, which streamline the analytical workflow, lower background interference, and enhance selectivity and specificity.

Keywords: Glycopeptides; MRM; PRM.

MeSH terms

Glycomics / methods
Lipidomics
Mass Spectrometry / methods
Metabolomics*
Proteomics* / methods