New insights into the chromosomes of stoneflies: I. Karyotype, C-banding and localization of ribosomal and telomeric DNA markers in Skwalacompacta (McLachlan, 1872) (Polyneoptera, Plecoptera, Perlodidae) from Siberia

Alexander Bugrov; Tatyana Karamysheva; Olesya Buleu

doi:10.3897/compcytogen.18.115784

New insights into the chromosomes of stoneflies: I. Karyotype, C-banding and localization of ribosomal and telomeric DNA markers in Skwalacompacta (McLachlan, 1872) (Polyneoptera, Plecoptera, Perlodidae) from Siberia

Comp Cytogenet. 2024 Jan 25:18:15-26. doi: 10.3897/compcytogen.18.115784. eCollection 2024.

Authors

Alexander Bugrov^{1

2}, Tatyana Karamysheva^{1

3}, Olesya Buleu^{1

2

3}

Affiliations

¹ Novosibirsk State University, Pirogova Str. 2, Novosibirsk 630090, Russia Novosibirsk State University Novosibirsk Russia.
² Institute of Systematics and Ecology of Animals, Russian Academy of Sciences, Siberian Branch, Frunze str. 11, 630091, Novosibirsk, Russia Institute of Systematics and Ecology of Animals, Russian Academy of Sciences Novosibirsk Russia.
³ Institute of Cytology and Genetics, Russian Academy of Sciences, Siberian Branch, Pr. Lavrentjeva 10, 630090, Novosibirsk, Russia Institute of Cytology and Genetics, Russian Academy of Sciences Novosibirsk Russia.

Abstract

This study provides data on chromosome number (2n♂♀=26), sex determination mechanism (XY♂/XX♀), C-banding pattern, distribution of clusters of telomeric TTAGG repeats and 18S ribosomal DNA in the karyotype of the stonefly Skwalacompacta (McLachlan, 1872). For the first time in the history of stoneflies cytogenetics, we provide photos of the chromosomes of the Plecoptera insects. The karyotype of males and females of S.compacta consists of 12 pairs of autosomes. Three pairs of large autosomes and four pairs of medium-sized autosomes are subacrocentric. The remaining pairs of autosomes are small, with unclear morphology. Pericentromeric C-bands were revealed in all autosomes. The sex chromosomes are also subacrocentric. The short arms of X and Y chromosomes are entirely heterochromatic and are rich in ribosomal DNA sequences. In the X chromosome this arm is larger than in the Y chromosome. It is likely that this arm associated with the nucleolar organizer (NOR). Telomeric DNA (TTAGG)_n repeats were detected in the terminal regions of all chromosomes.

Keywords: 18S rDNA repeats; C-banding; FISH; Plecoptera; karyotypes; telomeric (TTAGG)n DNA repeats.

Alexander Bugrov, Tatyana Karamysheva, Olesya Buleu.