Study of selenium enrichment metabolomics in Bacillus subtilis BSN313 via transcriptome analysis

Asad Ullah; Xian Yin; Muhammad Naveed; Sadar Aslam; Malik Wajid Hussain Chan; Sun Bo; Fenghuan Wang; Bo Xu; Baocai Xu; Zhou Yu

doi:10.1002/bab.2562

Study of selenium enrichment metabolomics in Bacillus subtilis BSN313 via transcriptome analysis

Biotechnol Appl Biochem. 2024 Feb 4. doi: 10.1002/bab.2562. Online ahead of print.

Authors

Asad Ullah^{1

2

3}, Xian Yin^{1

2}, Muhammad Naveed^{1

2}, Sadar Aslam⁴, Malik Wajid Hussain Chan^{1

2}, Sun Bo^{1

2

5}, Fenghuan Wang^{1

2}, Bo Xu⁶, Baocai Xu^{1

2}, Zhou Yu^{1

2}

Affiliations

¹ Key Laboratory of Geriatric Nutrition and Health (Beijing Technology and Business University), Ministry of Education, Beijing, China.
² China Food Flavor and Nutrition Health Innovation Center, Beijing Technology and Business University, Beijing, China.
³ Food and Marine Resources Research Center, Pakistan Council of Scientific and Industrial Research Laboratories Complex, Karachi, Pakistan.
⁴ State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.
⁵ Academy of National Food and Strategic Reserves Administration, Beijing, China.
⁶ McIntire School of Commerce, University of Virginia, Charlottesville, Virginia, USA.

PMID: 38311980
DOI: 10.1002/bab.2562

Abstract

In this study, the transcriptome analysis was practiced to identify potential genes of probiotic Bacillus subtilis BSN313 involved in selenium (Se) enrichment metabolism. The transcriptomic variation of the strain was deliberated in presence of three different sodium selenite concentrations (0, 3, and 20 μg/mL). The samples were taken at 1 and 13 h subsequent to inoculation of selenite and gene expression profiles in Se metabolism were analyzed through RNA sequencing. The gene expression levels of the pre log phase were lower than the stationary phase. It is because, the bacteria has maximum grown with high concentration of Se (enriched with organic Se), at stationary phase. Bacterial culture containing 3 μg/mL concentration of inorganic Se (sodium selenite) has shown highest gene expression as compared to no or high concentration of Se. This concentration (3 μg/mL) of sodium selenite (as Se) in the medium promoted the upregulation of thioredoxin reductase expression, whereas its higher Se concentration inhibited the formation of selenomethionine (SeMet). The result of 5 L bioreactor fermentation showed that SeMet was also detected in the fermentation supernatant as the growth entered in the late stationary phase and reached up to 857.3 ng/mL. The overall intracellular SeMet enriched content in BSN313 was extended up to 23.4 μg/g dry cell weight. The other two selenoamino acids (Se-AAs), methyl-selenocysteine, and selenocysteine were hardly detected in medium supernatant. From this study, it was concluded that SeMet was the highest content of organic Se byproduct biosynthesized by B. subtilis BSN313 strain in Se-enriched medium during stationary phase. Thus, B. subtilis BSN313 can be considered a commercial probiotic strain that can be used in the food and pharmaceutical industries. This is because it can meet the commercial demand for Se-AAs (SeMet) in both industries.

Keywords: Bacillus subtilis BNS313; methyl-selenocysteine; selenium enriched; selenoamino acids; selenocysteine; selenomethionine.

Abstract

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