Identification and expression analysis of expansin gene family in Salvia miltiorrhiza

Yunyun Li; Bin Li; Qiyue Pang; Yaoyu Lou; Donghao Wang; Zhezhi Wang

doi:10.1186/s13020-023-00867-w

Identification and expression analysis of expansin gene family in Salvia miltiorrhiza

Chin Med. 2024 Feb 4;19(1):22. doi: 10.1186/s13020-023-00867-w.

Authors

Yunyun Li¹, Bin Li^{1

2}, Qiyue Pang¹, Yaoyu Lou¹, Donghao Wang³, Zhezhi Wang⁴

Affiliations

¹ Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an, 710062, China.
² Xi'an Botanical Garden of Shaanxi Province (Institute of Botany of Shaanxi Province), Xi'an, China.
³ Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an, 710062, China. wangdonghao@snnu.edu.cn.
⁴ Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an, 710062, China. zzwang@snnu.edu.cn.

Abstract

Background: Expansins (EXP) are important enzymes that are involved in the extension of plant cells and regulation of root configurations, which play important roles in resisting various stresses. As a model medicinal plant, Salvia miltiorrhiza is well recognized for treating coronary heart disease, myocardial infection, and other cardiovascular and cerebrovascular diseases; however, the SmEXP gene family has not yet been analyzed.

Methods: The SmEXP family was systematically analyzed using bioinformatics. Quantitative real-time PCR was employed to analyze the tissue expression patterns of the SmEXP family, as well as its expression under abscisic acid (ABA) treatment and abiotic stress. Subcellular localization assay revealed the localization of SmEXLA1, SmEXLB1, and SmEXPA2.

Results: This study identified 29 SmEXP that belonged to four different subfamilies. SmEXP promoter analysis suggested that it may be involved in the growth, development, and stress adaptation of S. miltiorrhiza. An analysis of the expression patterns of SmEXP revealed that ABA, Cu²⁺, and NaCl had regulatory effects on its expression. A subcellular localization assay showed that SmEXLA1 and SmEXLB1 were located on the nucleus and cell membrane, while SmEXPA2 was located on the cell wall.

Conclusion: For this study, the SmEXP family was systematically analyzed for the first time, which lays a foundation for further elucidating its physiological and biological functionality.

Keywords: ABA; Abiotic stress; Bioinformatics; Expansin; Salvia miltiorrhiza; Subcellular localization.

Abstract

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