Integrated biological system for remediation and valorization of tannery wastewater: Focus on microbial communities responsible for methanogenesis and sulfidogenesis

Abstract

Microbial communities in hybrid linear flow channel reactors and anaerobic sequencing batch reactors operated in series for remediation and beneficiation of tannery wastewater were assessed. Despite concurrent sulfidogenesis, more intensive pre-treatment in hybrid linear flow channel reactors reduced methanogenic inhibition usually associated with anaerobic digestion of tannery effluent and promoted efficiency (max 321 mLCH₄/gCOD_consumed, 59% biogas CH₄). Nitrification and biological sulfate reduction were key metabolic pathways involved in overall and sulfate reducing bacterial community selection, respectively, during pre-treatment. Taxonomic selection could be explained by the proteinaceous and saline character of tannery effluent, with dominant genera being protein and/or amino acid degrading, halotolerant and/or ammonia tolerant. Complete oxidizers dominated the sulfidogenic populations during pre-treatment, while aceticlastic genera dominated the methanogenic populations during anaerobic digestion. With more intensive pre-treatment, the system shows promise for remediation and recovery of biogas and sulfur from tannery wastewater in support of a bio-circular economy.

Keywords: Anaerobic sequencing batch reactor; Hybrid linear flow channel reactor; Methanogenic archaea; Sulfate reducing bacteria.