Association of exosomes in patients with compromised myocardial perfusion on functional imaging

Chia-Ju Liu; Jien-Jiun Chen; Jo-Hsuan Wu; Yao-Te Chung; Jin-Wun Chen; Meng-Tsun Liu; Chu-Hsuan Chiu; Yi-Cheng Chang; Sheng-Nan Chang; Jou-Wei Lin; Juey-Jen Hwang

doi:10.1016/j.jfma.2024.01.019

Association of exosomes in patients with compromised myocardial perfusion on functional imaging

J Formos Med Assoc. 2024 Feb 1:S0929-6646(24)00052-4. doi: 10.1016/j.jfma.2024.01.019. Online ahead of print.

Authors

Affiliations

¹ Department of Nuclear Medicine, National Taiwan University Hospital, Taipei City, Taiwan.
² Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yun-Lin Branch, Dou-Liu City, Taiwan.
³ Shiley Eye Institute and Viterbi Family Department of Ophthalmology, University of California, San Diego, CA, USA.
⁴ Graduate Institute of Medical Genomics and Proteomics, Taipei, Taiwan; Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
⁵ Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yun-Lin Branch, Dou-Liu City, Taiwan. Electronic address: p95421008@ntu.edu.tw.
⁶ Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yun-Lin Branch, Dou-Liu City, Taiwan; Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.

PMID: 38307800
DOI: 10.1016/j.jfma.2024.01.019

Abstract

Objectives: Exosomes are membrane vesicles that are actively secreted in response to microenvironmental stimuli. In this study, we quantified the amount of exosomes in patients with significant coronary artery disease (CAD) and evaluated its relationship with myocardial perfusion imaging (MPI) results.

Methods: Patients who underwent both MPI and coronary angiography were recruited. Plasma was collected during angiography, and exosomes were extracted via the precipitation method. The summed stress scores (SSS), summed difference scores, and ventricular functional parameters were calculated from the MPI and compared with the amounts of exosomes and extracted miRNAs.

Results: In total, 115 patients were enrolled (males: 78 %; mean age: 66.6 ± 10.6 years). Those with abnormal SSS according to the MPI had significantly fewer exosomes (p = 0.032). After multivariate analysis, the SSS remained significantly related to the amount of exosomes (p = 0.035). In forty randomly selected samples, miRNA-432-5p and miRNA-382-3p were upregulated in patients with abnormal SSS.

Conclusions: Patients with compromised poststress myocardial perfusion on MPI tended to have fewer exosomes in association with CAD-related miRNAs. This is the first study to clarify the fundamental and pathophysiological causes of CAD using radiographic examinations.

Keywords: Coronary artery disease (CAD); Exosomes; MiRNA; Myocardial perfusion imaging (MPI).