Extracellular matrix protein composition dynamically changes during murine forelimb development

Kathryn R Jacobson; Aya M Saleh; Sarah N Lipp; Chengzhe Tian; Audrey R Watson; Callan M Luetkemeyer; Alexander R Ocken; Sabrina L Spencer; Tamara L Kinzer-Ursem; Sarah Calve

doi:10.1016/j.isci.2024.108838

Extracellular matrix protein composition dynamically changes during murine forelimb development

iScience. 2024 Jan 9;27(2):108838. doi: 10.1016/j.isci.2024.108838. eCollection 2024 Feb 16.

Authors

Kathryn R Jacobson^{1

2}, Aya M Saleh³, Sarah N Lipp^{3

4}, Chengzhe Tian^{5

6

7}, Audrey R Watson^{5

6}, Callan M Luetkemeyer⁸, Alexander R Ocken³, Sabrina L Spencer^{5

6}, Tamara L Kinzer-Ursem^{1

3}, Sarah Calve^{1

3

6

8}

Affiliations

¹ Purdue University Interdisciplinary Life Science Program, Purdue University, West Lafayette, IN 47907, USA.
² Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA.
³ Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.
⁴ The Indiana University Medical Scientist/Engineer Training Program, Indiana University, Indianapolis, IN 46202, USA.
⁵ Department of Biochemistry, University of Colorado Boulder, Boulder, CO 80303, USA.
⁶ BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80303, USA.
⁷ Research Center for Molecular Medicine (CEMM) of the Austrian Academy of Sciences, Vienna, Austria.
⁸ Paul M. Rady Department of Mechanical Engineering, University of Colorado Boulder, Boulder, CO 80309, USA.

Abstract

The extracellular matrix (ECM) is an integral part of multicellular organisms, connecting different cell layers and tissue types. During morphogenesis and growth, tissues undergo substantial reorganization. While it is intuitive that the ECM remodels in concert, little is known regarding how matrix composition and organization change during development. Here, we quantified ECM protein dynamics in the murine forelimb during appendicular musculoskeletal morphogenesis (embryonic days 11.5-14.5) using tissue fractionation, bioorthogonal non-canonical amino acid tagging, and mass spectrometry. Our analyses indicated that ECM protein (matrisome) composition in the embryonic forelimb changed as a function of development and growth, was distinct from other developing organs (brain), and was altered in a model of disease (osteogenesis imperfecta murine). Additionally, the tissue distribution for select matrisome was assessed via immunohistochemistry in the wild-type embryonic and postnatal musculoskeletal system. This resource will guide future research investigating the role of the matrisome during complex tissue development.

Keywords: Biological sciences; Developmental biology; Natural sciences.

Abstract

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