Electron microscopic observation of Mycobacterium avium subsp. paratuberculosis in cattle feces after a novel purification using liquid paraffin

Masatoshi Fujihara; Daisuke Kondoh; Shigeru Matsuzawa; Ikunori Naito

doi:10.1016/j.mimet.2024.106891

Electron microscopic observation of Mycobacterium avium subsp. paratuberculosis in cattle feces after a novel purification using liquid paraffin

J Microbiol Methods. 2024 Feb-Mar:217-218:106891. doi: 10.1016/j.mimet.2024.106891. Epub 2024 Jan 30.

Authors

Masatoshi Fujihara¹, Daisuke Kondoh², Shigeru Matsuzawa³, Ikunori Naito⁴

Affiliations

¹ Hokkaido Kushiro Livestock Hygiene Service Center, Kushiro, Hokkaido, Japan. Electronic address: fujihara.masatoshi@pref.hokkaido.lg.jp.
² Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan. Electronic address: kondoh-d@obihiro.ac.jp.
³ Hokkaido Kushiro Livestock Hygiene Service Center, Kushiro, Hokkaido, Japan. Electronic address: matsuzawa.shigeru@pref.hokkaido.lg.jp.
⁴ Local Independent Administrative Agency Hokkaido Research Organization, Shintoku, Hokkaido, Japan. Electronic address: naitou-ikunori@hro.or.jp.

PMID: 38302060
DOI: 10.1016/j.mimet.2024.106891

Abstract

We developed a novel method for purifying acid-fast bacteria from feces. The method enabled the observation of characteristic clumps of Mycobacterium avium subsp. paratuberculosis (MAP) under electron microscopy by removing contaminants and other bacteria. Further refinement of this method will contribute to efficient and effective MAP detection.

Keywords: Clump; Electron microscopy; Mycobacterium avium subsp. paratuberculosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Cattle Diseases* / diagnosis
Cattle Diseases* / microbiology
Electrons
Feces / microbiology
Mineral Oil
Mycobacterium avium subsp. paratuberculosis*
Paratuberculosis* / diagnosis
Paratuberculosis* / microbiology

Substances

Mineral Oil