Pyrosequencing analysis of bacterial community changes in dental unit waterlines after chlorogenic acid treatment

Na Li; Qin-Ming Cai; Ni-Ya Hu; Shu-Ling Jiang; Fu-Qing Chen; Qiao-Qiao Hu; Fen Yang; Chao-Zhu He

doi:10.3389/fcimb.2024.1303099

Pyrosequencing analysis of bacterial community changes in dental unit waterlines after chlorogenic acid treatment

Front Cell Infect Microbiol. 2024 Jan 17:14:1303099. doi: 10.3389/fcimb.2024.1303099. eCollection 2024.

Authors

Na Li^{1

2}, Qin-Ming Cai^{3

4}, Ni-Ya Hu³, Shu-Ling Jiang^{1

2}, Fu-Qing Chen¹, Qiao-Qiao Hu¹, Fen Yang¹, Chao-Zhu He²

Affiliations

¹ Department of Stomatology, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, China.
² Nursing School, Nanchang University, Nanchang, China.
³ The First Affiliated Hospital of Nanchang University, School of Public Health, Nanchang University, Nanchang, China.
⁴ The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China.

Abstract

Introduction: The contamination of dental unit waterlines (DUWLs) poses a significant risk of cross-infection in dentistry. Although chemical disinfectants have been effective in reducing number of bacteria, they do have limitations.

Methods: This study aimed to investigate the potential of chlorogenic acid, a natural substance with broadspectrum antibacterial properties, for treating DUWLs. Over a period of three months, we analyzed the microbial communities in 149 DUWLs samples collected from 5 dental units using high-throughput pyrophosphate sequencing.

Results: The results revealed that chlorogenic acid treatment had a significant impact on the microbial community profile in the DUWLs, with the most significant changes occurring within the first 15 days and stabilization observed in the last 30 days. The predominant genera detected in the samples were Bacteroides, Lactobacillus, Streptococcus, Methylobacterium, and Phreatobacter. Additionally, the relative abundance of certain beneficial bacteria, such as Alloprevotella, Roseburia, and Blautia, increased, while the presence of opportunistic pathogens like Mycobacteria significantly decreased. The functional prediction analysis using the KEGG database indicated a decrease in the pathogenicity of the bacterial community in the DUWLs following chlorogenic acid treatment.

Discussion: This study introduces a novel approach for the prevention and treatment of infections associated with dental care.

Keywords: bacterial community; chlorogenic acid; clinical practice-related infections; dental unit waterlines; pyrosequencing analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria
Biofilms
Chlorogenic Acid* / pharmacology
Colony Count, Microbial
Equipment Contamination* / prevention & control
High-Throughput Nucleotide Sequencing
Water Microbiology

Substances

Chlorogenic Acid

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. The present study was supported by grants from the Scientific Project of Jiangxi Province (grant no.20224BAB206054) and the Science and Technology Program of Health and Family Planning Commission of Jiangxi province Scientific Research (grant no. 202310026).