Does YAP influence cell proliferation and apoptosis in benign epithelial odontogenic lesions?

Rani Iani Costa Gonçalo; Julliany Taverny Sousa; Carla Samily de Oliveira Costa; Rodrigo Porpino Mafra; Janaina Lessa de Moraes Dos Santos; Leorik Pereira da Silva; Lélia Maria Guedes Queiroz

doi:10.1111/odi.14882

Does YAP influence cell proliferation and apoptosis in benign epithelial odontogenic lesions?

Oral Dis. 2024 Jan 31. doi: 10.1111/odi.14882. Online ahead of print.

Authors

Rani Iani Costa Gonçalo¹, Julliany Taverny Sousa¹, Carla Samily de Oliveira Costa¹, Rodrigo Porpino Mafra¹, Janaina Lessa de Moraes Dos Santos¹, Leorik Pereira da Silva², Lélia Maria Guedes Queiroz¹

Affiliations

¹ Department of Oral Pathology, Federal University of Rio Grande do Norte, Natal, Brazil.
² Oral Histopathology Service, Federal University of Campina Grande, Patos, PB, Brazil.

PMID: 38297810
DOI: 10.1111/odi.14882

Abstract

Objective: To analyze the immunohistochemical expression of YAP and its correlation with markers involved in cell proliferation and apoptosis in benign epithelial odontogenic lesions.

Study design: The sample consisted of 95 cases of odontogenic lesions (25 dentigerous cysts, 30 non-syndromic odontogenic keratocysts, 30 conventional ameloblastomas, and 10 unicystic ameloblastomas) and 10 dental follicles used as normal odontogenic tissue. The histological sections were submitted to immunohistochemistry with YAP, cyclin D1, Ki-67, and Bcl-2 antibodies. Immunoexpression was analyzed qualitatively and quantitatively using an adapted method. The collected data were analyzed descriptively and statistically (p ≤ 0.05).

Results: The highest YAP expression was observed in odontogenic keratocysts, followed by unicystic ameloblastomas and conventional ameloblastomas, which exhibited moderate immunoreactivity predominantly in peripheral cells. Furthermore, significant differences in YAP immunoexpression were observed between the groups analyzed, with significant positive correlations between YAP and cyclin D1 in dentigerous cysts and unicystic ameloblastomas and between YAP and Ki-67 in unicystic ameloblastomas (p < 0.05). However, there were no statistically significant correlations between YAP and Bcl-2 immunoexpression in the groups studied.

Conclusion: YAP may influence epithelial cell proliferation in odontogenic cysts and tumors, suggesting its possible participation in the progression of the odontogenic lesions studied.

Keywords: apoptosis; cell proliferation; immunohistochemistry; odontogenic cysts; odontogenic tumors; yes-associated protein.