SERS-based detection of the antibiotic ceftriaxone in spiked fresh plasma and microdialysate matrix by using silver-functionalized silicon nanowire substrates

Chen Liu; Célia Franceschini; Susanne Weber; Tony Dib; Poting Liu; Long Wu; Edoardo Farnesi; Wen-Shu Zhang; Vladimir Sivakov; Peter B Luppa; Jürgen Popp; Dana Cialla-May

doi:10.1016/j.talanta.2024.125697

SERS-based detection of the antibiotic ceftriaxone in spiked fresh plasma and microdialysate matrix by using silver-functionalized silicon nanowire substrates

Talanta. 2024 May 1:271:125697. doi: 10.1016/j.talanta.2024.125697. Epub 2024 Jan 18.

Authors

Affiliations

¹ Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Albert-Einstein-Straße 9, 07745, Jena, Germany; Institute of Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich Schiller University Jena, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Helmholtzweg 4, 07743, Jena, Germany.
² UR Molecular Systems, Department of Chemistry, University of Liège, 4000, Liège, Belgium.
³ Institute of Clinical Chemistry and Pathobiochemistry, Klinikum Rechts der Isar of the Technische Universität München, Ismaninger Str. 22, 81675, München, Germany.
⁴ Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Albert-Einstein-Straße 9, 07745, Jena, Germany; School of Food Science and Engineering, Key Laboratory of Tropical and Vegetables Quality and Safety for State Market Regulation, Hainan University. Haikou 570228, China; Key Laboratory of Fermentation Engineering (Ministry of Education), College of Bioengineering and Food, Hubei University of Technology, Wuhan, 430068, China.
⁵ China Fire and Rescue Institute, Beijing, 102202, China.
⁶ Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Albert-Einstein-Straße 9, 07745, Jena, Germany.
⁷ Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Albert-Einstein-Straße 9, 07745, Jena, Germany; Institute of Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich Schiller University Jena, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Helmholtzweg 4, 07743, Jena, Germany. Electronic address: dana.cialla-may@leibniz-ipht.de.

PMID: 38295449
DOI: 10.1016/j.talanta.2024.125697

Abstract

Therapeutic drug monitoring (TDM) is an important tool in precision medicine as it allows estimating pharmacodynamic and pharmacokinetic effects of drugs in clinical settings. An accurate, fast and real-time determination of the drug concentrations in patients ensures fast decision-making processes at the bedside to optimize the clinical treatment. Surface-enhanced Raman spectroscopy (SERS), which is based on the application of metallic nanostructured substrates to amplify the inherent weak Raman signal, is a promising technique in medical research due to its molecular specificity and trace sensitivity accompanied with short detection times. Therefore, we developed a SERS-based detection scheme using silicon nanowires decorated with silver nanoparticles, fabricated by means of top-down etching combined with chemical deposition, to detect the antibiotic ceftriaxone (CRO) in spiked fresh plasma and microdialysis samples. We successfully detected CRO in both matrices with an LOD of 94 μM in protein-depleted fresh plasma and 1.4 μM in microdialysate.

Keywords: Ceftriaxone; Fresh plasma; Microdialysate; Nanoparticles; Quantitative analysis; SERS; Silicon nanowires; Silver; TDM.

MeSH terms

Anti-Bacterial Agents / pharmacology
Ceftriaxone
Humans
Metal Nanoparticles* / chemistry
Nanowires* / chemistry
Silicon / chemistry
Silver / chemistry
Spectrum Analysis, Raman / methods

Substances

Anti-Bacterial Agents
Silver
Ceftriaxone
Silicon