Interleukin 6 polymorphisms are associated with cardiovascular risk factors in premature coronary artery disease patients and healthy controls of the GEA Mexican study

Rosalinda Posadas-Sánchez; Ángel Rene López-Uribe; José Manuel Fragoso; Gilberto Vargas-Alarcón

doi:10.1016/j.yexmp.2024.104886

Interleukin 6 polymorphisms are associated with cardiovascular risk factors in premature coronary artery disease patients and healthy controls of the GEA Mexican study

Exp Mol Pathol. 2024 Apr:136:104886. doi: 10.1016/j.yexmp.2024.104886. Epub 2024 Feb 1.

Authors

Rosalinda Posadas-Sánchez¹, Ángel Rene López-Uribe¹, José Manuel Fragoso², Gilberto Vargas-Alarcón³

Affiliations

¹ Department of Endocrinology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico.
² Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico.
³ Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico; Research Direction, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico. Electronic address: gilberto.vargas@cardiologia.org.mx.

PMID: 38290570
DOI: 10.1016/j.yexmp.2024.104886

Abstract

Background and aims: Interleukin-6 (IL-6) is an acute-phase protein that plays an important role in the inflammatory response, vascular inflammation, and atherosclerosis process. The study aimed to establish whether IL-6 gene polymorphisms and IL-6 concentrations are associated with premature coronary artery disease (pCAD) and cardiovascular risk factors.

Methods: The IL-6 concentrations and the rs2069827, rs1800796, and rs1800795 IL-6 polymorphisms were determined in 1150 pCAD patients and 1083 healthy controls (coronary artery calcium equal to zero determined by tomography).

Results: The IL-6 polymorphisms studied were not associated with pCAD, but they were associated with cardiovascular risk factors in patients and controls. In controls, under the dominant model, the rs1800795 C allele and the rs2069827 T allele were associated with a low risk of central obesity (OR = 0.401, p = 0.017 and OR = 0.577, p = 0.031, respectively), hypoalphalipoproteinemia (OR = 0.581, p = 0.027 and OR = 0.700, p = 0.014, respectively) and hypertriglyceridemia (OR = 0.575, p = 0.030 and OR = 0.728, p = 0.033, respectively). In pCAD, the rs1800795 C allele was associated with an increased risk of hypoalphalipoproteinemia (OR = 1.370, p_additive = 0.025) and increased C-reactive protein (CRP) concentrations (OR = 1.491, p_additive = 0.007). pCAD patients had significantly higher serum IL-6 concentrations compared to controls (p = 0.002). In the total population, individuals carrying the rs1800795 GC + CC genotypes had higher levels of IL-6 than carriers of the GG genotype (p = 0.025). In control individuals carrying the C allele (CG + CC), an inverse correlation was observed between IL-6 and HDL-cholesterol levels (p = 0.003).

Conclusions: In summary, the IL-6 polymorphisms were not associated with pCAD, however, they were associated with cardiovascular risk factors in pCAD patients and healthy controls. Individuals carrying the rs1800795 GC + CC genotypes had higher levels of IL-6 than carriers of the GG genotype.

Keywords: Atherosclerosis; Coronary artery disease; Interleukin 6; Polymorphisms; Susceptibility.

MeSH terms

Cardiovascular Diseases* / genetics
Case-Control Studies
Coronary Artery Disease* / genetics
Genetic Predisposition to Disease
Heart Disease Risk Factors
Humans
Hypoalphalipoproteinemias*
Interleukin-6* / genetics
Polymorphism, Single Nucleotide
Risk Factors

Substances

Interleukin-6
IL6 protein, human