Does poly-3-hydroxybutyrate biodegradation affect the quality of soil organic matter?

Abstract

The search for eco-friendly substitutes for traditional plastics has led to the production of biodegradable bioplastics. However, concerns have been raised about the impact of bioplastic biodegradation on soil health. Despite these concerns, the potential negative consequences of bioplastics during various stages of biodegradation remain underexplored. Therefore, this study aims to investigate the impact of micro-bioplastics made of poly-3-hydroxybutyrate (P3HB) on the properties of three different soils. In our ten-month experiment, we investigated the impact of poly-3-hydroxybutyrate (P3HB) on Chernozem, Cambisol, and Phaeozem soils. Our study focused on changes in soil organic matter (SOM), microbial activity, and the level of soil carbon and nitrogen. The observed changes indicated an excessive level of biodegradation of SOM after the soils were enriched with micro-particles of P3HB, with concentrations ranging from 0.1% to 3%. The thermogravimetric analysis confirmed the presence of residual P3HB (particularly in the 3% treatment) and underscored the heightened biodegradation of SOM, especially in the more stable SOM fractions. This was notably evident in Phaeozem soils, where even the stable SOM pool was affected. Elemental analysis revealed changes in soil organic carbon content following P3HB degradation, although nitrogen levels remained constant. Enzymatic activity was found to vary with soil type and responded differently across P3HB concentration levels. Our findings confirmed that P3HB acts as a bioavailable carbon source. Its biodegradation stimulates the production of enzymes, which in turn affects various soil elements, indicating complex interactions within the soil ecosystem.