A Stereological Approach to Quantify Immunohistochemical Staining in Zebrafish Larvae

Dércia Santos; Sandra M Monteiro; Ana Luzio

doi:10.1007/978-1-0716-3625-1_23

A Stereological Approach to Quantify Immunohistochemical Staining in Zebrafish Larvae

Methods Mol Biol. 2024:2753:397-402. doi: 10.1007/978-1-0716-3625-1_23.

Authors

Dércia Santos^{1

2}, Sandra M Monteiro^{1

2}, Ana Luzio^{3

4}

Affiliations

¹ Centre for the Research and Technology of Agro-Environmental and Biological Sciences, CITAB and Inov4Agro, Institute for Innovation, Capacity Building and Sustainability of Agri-food Production, Quinta de Prados, Vila Real, Portugal.
² University of Trás-os-Montes and Alto Douro, Quinta de Prados, Vila Real, Portugal.
³ Centre for the Research and Technology of Agro-Environmental and Biological Sciences, CITAB and Inov4Agro, Institute for Innovation, Capacity Building and Sustainability of Agri-food Production, Quinta de Prados, Vila Real, Portugal. aluzio@utad.pt.
⁴ University of Trás-os-Montes and Alto Douro, Quinta de Prados, Vila Real, Portugal. aluzio@utad.pt.

PMID: 38285354
DOI: 10.1007/978-1-0716-3625-1_23

Abstract

Immunohistochemistry (IHC) is a powerful research tool to localize specific antigens in whole-mount or tissue sections of embryos with labeled antibodies based on antigen-antibody interactions. Stereological methods are nowadays an essential tool to quantify cells or other types of structures in an unbiased and reproducible manner. In this chapter, a general protocol for a stereological estimation of the relative volume density of each structural component (Vv), which can be applied to any organ/structural component, will be described.

Keywords: Absolute volume (V); Histological techniques; Point-counting; Relative volume density (Vv).

MeSH terms

Animals
Antibodies
Larva
Perciformes*
Staining and Labeling
Zebrafish*

Substances

Antibodies