Inhibition of CX3CL1 by treadmill training prevents osteoclast-induced fibrocartilage complex resorption during TBI healing

Xiao Liu; Mei Zhou; Jindong Tan; Lin Ma; Hong Tang; Gang He; Xu Tao; Lin Guo; Xia Kang; Kanglai Tang; Xuting Bian

doi:10.3389/fimmu.2023.1295163

Inhibition of CX3CL1 by treadmill training prevents osteoclast-induced fibrocartilage complex resorption during TBI healing

Front Immunol. 2024 Jan 12:14:1295163. doi: 10.3389/fimmu.2023.1295163. eCollection 2023.

Authors

Xiao Liu^#¹, Mei Zhou^#¹, Jindong Tan¹, Lin Ma¹, Hong Tang¹, Gang He¹, Xu Tao¹, Lin Guo¹, Xia Kang², Kanglai Tang¹, Xuting Bian¹

Affiliations

¹ Department of Sports Medicine, Southwest Hospital, Army Medical University, Chongqing, China.
² Pancreatic Injury and Repair Key Laboratory of Sichuan Province, The General Hospital of Western Theater Command, Chengdu, Sichuan, China.

^# Contributed equally.

Abstract

Introduction: The healing of tendon-bone injuries is very difficult, often resulting in poor biomechanical performance and unsatisfactory functional recovery. The tendon-bone insertion has a complex four distinct layers structure, and previous studies have often focused on promoting the regeneration of the fibrocartilage layer, neglecting the role of its bone end repair in tendon-bone healing. This study focuses on the role of treadmill training in promoting bone regeneration at the tendon-bone insertion and its related mechanisms.

Methods: After establishing the tendon-bone insertion injury model, the effect of treadmill training on tendon-bone healing was verified by Micro CT and HE staining; then the effect of CX3CL1 on osteoclast differentiation was verified by TRAP staining and cell culture; and finally the functional recovery of the mice was verified by biomechanical testing and behavioral test.

Results: Treadmill training suppresses the secretion of CX3CL1 and inhibits the differentiation of local osteoclasts after tendon-bone injury, ultimately reducing osteolysis and promoting tendon bone healing.

Discussion: Our research has found the interaction between treadmill training and the CX3CL1-C3CR1 axis, providing a certain theoretical basis for rehabilitation training.

Keywords: CX3CL1; CX3CR1; bone eminences; osteoclast; tendon-bone insertion healing; treadmill training.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Resorption* / prevention & control
Chemokine CX3CL1* / physiology
Fibrocartilage / physiology
Mice
Osteoclasts
Physical Conditioning, Animal*
Tendon Injuries*
Tendons
Wound Healing

Substances

Cx3cl1 protein, mouse
Chemokine CX3CL1

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This study was funded by the Key Projects of the Natural Science Foundation of China (Grant No. 82130071).