Lipopolysaccharides of Herbaspirillum species and their relevance for bacterium-host interactions

Natalya S Velichko; Maxim S Kokoulin; Pavel S Dmitrenok; Vyacheslav S Grinev; Polina D Kuchur; Aleksey S Komissarov; Yulia P Fedonenko

doi:10.1016/j.ijbiomac.2024.129516

Lipopolysaccharides of Herbaspirillum species and their relevance for bacterium-host interactions

Int J Biol Macromol. 2024 Mar;261(Pt 1):129516. doi: 10.1016/j.ijbiomac.2024.129516. Epub 2024 Jan 24.

Authors

Natalya S Velichko¹, Maxim S Kokoulin², Pavel S Dmitrenok², Vyacheslav S Grinev³, Polina D Kuchur⁴, Aleksey S Komissarov⁴, Yulia P Fedonenko⁵

Affiliations

¹ Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov 410049, Russia. Electronic address: velichko_n@ibppm.ru.
² G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, 159 Prospekt 100 Let Vladivostoku, Vladivostok 690022, Russia.
³ Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov 410049, Russia; Saratov State University, 83 Ulitsa Astrakhanskaya, Saratov 410012, Russia.
⁴ Applied Genomics Laboratory, SCAMT Institute, ITMO University, 9 Ulitsa Lomonosova, St. Petersburg 191002, Russia.
⁵ Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov 410049, Russia; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, 159 Prospekt 100 Let Vladivostoku, Vladivostok 690022, Russia.

PMID: 38278393
DOI: 10.1016/j.ijbiomac.2024.129516

Abstract

The lipopolysaccharides of Herbaspirillum lusitanum P6-12^T (HlP6-12^T) and H. frisingense GSF30^T (HfGSF30^T) was isolated by phenol-water extraction from bacterial cells and was characterized using chemical analysis and SDS-PAGE. It was shown that these bacteria produce LPSs that differ in their physicochemical properties and macromolecular organization. In this paper, the lipid A structure of the HlP6-12^T LPS, was characterized through chemical analyses and matrix-assisted laser desorption ionization (MALDI) mass spectrometry. To prove the effect of the size of micelles on their bioavailability, we examined the activity of both LPSs toward the morphology of wheat seedlings. Analysis of the HlP6-12^T and HfGSF30^T genomes showed no significant differences between the operons that encode proteins involved in the biosynthesis of the lipids A and core oligosaccharides. The difference may be due to the composition of the O-antigen operon. HfGSF30^T has two copies of the rfb operon, with the main one divided into two fragments. In contrast, the HlP6-12^T genome contains only a single rfb-containing operon, and the other O-antigen operons are not comparable at all. The integrity of O-antigen-related genes may also affect LPS variability of. Specifically, we have observed a hairpin structure in the middle of the O-antigen glycosyltransferase gene, which led to the division of the gene into two fragments, resulting in incorrect protein synthesis and potential abnormalities in O-antigen production.

Keywords: Antioxidant enzymes; Herbaspirillum; Lipid A; Lipopolysaccharide (LPS); O-antigen genes.

MeSH terms

Gas Chromatography-Mass Spectrometry
Herbaspirillum* / genetics
Host Microbial Interactions
Lipopolysaccharides* / chemistry
O Antigens / metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

Lipopolysaccharides
O Antigens