Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis System

Abstract

Antibodies specifically recognizing integral membrane proteins are essential tools for functional analysis, diagnosis, and therapeutics targeting membrane proteins. However, developing antibodies against membrane proteins remains a big challenge because mass production of membrane proteins is difficult. Recently, we developed a highly efficient cell-free production method of proteoliposome antigen using a cell-free protein synthesis method with liposome and dialysis cup. Here, we introduce practical and efficient integrated procedures to produce a large amount of proteoliposome antigen for anti-membrane protein antibody development.

Keywords: Adjuvant; Cell-free protein synthesis; Immunizing antigen; Membrane protein; Proteoliposome.