Accurate, timely human immunodeficiency virus (HIV) diagnosis is critical. Routine HIV screening program data were examined before and after reflex HIV type 1 RNA testing. Reflex testing facilitated confirmation of reactive HIV screening assays (as true or false positives) (odds ratio, 23.7 [95% confidence interval, 6.7-83.4]; P < .0001), improving detection of acute HIV and reducing unconfirmed discordant results.
Keywords: HIV testing; acute HIV infection; discordant results; reflex HIV-1 RNA.
© The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America.