Analysis of growth dynamics in five different media and metabolic phenotypic characteristics of Piriformospora indica

Jing-Rong Hu; Jin-Meng Li; Hai-Yan Wang; Mei-Li Sun; Chun-Yang Huang; Han-Cheng Wang

doi:10.3389/fmicb.2023.1301743

Analysis of growth dynamics in five different media and metabolic phenotypic characteristics of Piriformospora indica

Front Microbiol. 2024 Jan 8:14:1301743. doi: 10.3389/fmicb.2023.1301743. eCollection 2023.

Authors

Jing-Rong Hu^{1

2}, Jin-Meng Li², Hai-Yan Wang¹, Mei-Li Sun², Chun-Yang Huang³, Han-Cheng Wang⁴

Affiliations

¹ Institute of Advanced Agricultural Science, Hubei University of Arts and Science, Xiangyang, Hubei, China.
² College of Agriculture, Yangtze University, Jingzhou, China.
³ Guizhou Provincial Tobacco Company, Zunyi Branch, Zunyi, China.
⁴ Guizhou Provincial Academician Workstation of Microbiology and Health, Guizhou Academy of Tobacco Science, Guiyang, Guizhou, China.

Abstract

Piriformospora indica is an important endophytic fungus with broad potential for alleviating biotic and abiotic stress on host plants. This study monitored the growth dynamics of P. indica on five commonly used artificial media for microorganisms and analyzed its metabolic characteristics using Biolog Phenotype Microarray (PM) technology. The results showed that P. indica grew fastest on Potato Dextrose Agar (PDA), followed by Kidney Bean Agar (KBA), Alkyl Ester Agar (AEA), Oatmeal Agar (OA), and Luria-Bertani Agar (LB), and the most suitable medium for spore production was OA. Using Biolog PM1-10, 950 metabolic phenotypes of P. indica were obtained. P. indica could metabolize 87.89% of the tested carbon sources, 87.63% of the tested nitrogen sources, 96.61% of the tested phosphorus sources, and 100% of the tested sulfur sources. P. indica displayed 92 kinds of tested biosynthetic pathways, and it could grow under 92 kinds of tested osmotic pressures and 88 kinds of tested pH conditions. PM plates 1-2 revealed 43 efficient carbon sources, including M-Hydroxyphenyl acid, N-Acetyl-D-Glucosamine, Tyramine, Maltotrios, α-D-Glucosine, I-Erythritol, L-Valine, D-Melezitose, D-Tagatose, and Turanose. PM plates 3,6-8 indicated 170 efficient nitrogen sources, including Adenosine, Inosine Allantoin, D, L-Lactamide, Arg-Met, lle-Trp, Ala-Arg, Thr-Arg, Trp-Tyr, Val-Asn, Gly-Gly-D-Leu, Gly-Gly-Phe, and Leu-Leu-Leu. This study demonstrates that P. indica can metabolize a variety of substrates, such as carbon and nitrogen sources, and has a wide range of environmental adaptability. The growth dynamics on artificial culture media and metabolic phenotypes of P. indica can be used to investigate its biological characteristics, screen for more suitable growth and sporulation conditions, and elucidate the physiological mechanisms that enhance the stress resistance of host plants. This study provides a theoretical basis for its better application in agriculture.

Keywords: Biolog Phenotype Microarray; Piriformospora indica; culture media; growth and sporulation; metabolic profiling.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This study was supported by the Key R&D Project of the Zunyi Company of Guizhou Tobacco Company (2022XM09), the National Natural Science Foundation (Grant Nos. 32302343 and 32172400), the China National Tobacco Corporation [110202101048 (LS-08)], the Hundred Level Innovative Talent Foundation of Guizhou Province [GCC(2022)028-1], the Guizhou Science Technology Foundation (ZK[2021]Key036), and the Guizhou Province Applied Technology Research and Development Funding Postsubsidy Project. The authors declare that this study received funding from the Zunyi Company of Guizhou Tobacco Company and the China National Tobacco Corporation. The funders were not involved in the study design, collection, analysis, and interpretation of data, the writing of this article, or the decision to submit it for publication.