MXSGD alleviates CsA-induced hypoimmunity lung injury by regulating microflora metabolism

Front Immunol. 2024 Jan 8:14:1298416. doi: 10.3389/fimmu.2023.1298416. eCollection 2023.

Abstract

Context: Ma Xing Shi Gan Decoction (MXSGD) is a traditional remedy for treating lung injuries that was developed by the Typhoid and Fever School of Pharmaceutical Biology. It has antitussive and expectorant effects, anti-inflammatory, antiviral, regulates the body's immunity, etc.

Aim: The aim of this study is to investigate whether MXSGD can ameliorate cyclosporine A (CsA)-induced hypoimmunity lung injury by regulating microflora metabolism. Methods: Establishment of a model for CsA-induced hypoimmunity lung injury. Using 16S rRNA high-throughput sequencing and LC-MS, the effects of MXSGD on gut flora and lung tissue microecology of mice with CsA-induced hypoimmunity were investigated.

Results: MXSGD was able to preserve lung tissue morphology and structure, reduce serum inflammatory marker expression and protect against CsA-induced lung tissue damage. Compared to the model, MXSGD increased beneficial gut bacteria: Eubacterium ventriosum group and Eubacterium nodatum group; decreased intestinal pathogens: Rikenellaceae RC9 intestinal group; reduced the abundance of Chryseobacterium and Acinetobacter, promoted the production of Lactobacillus and Streptococcus, and then promoted the lung flora to produce short-chain fatty acids. MXSGD was able to enhance the expression of serum metabolites such as Americine, 2-hydroxyhexadecanoylcarnitine, Emetine, All-trans-decaprenyl diphosphate, Biliverdin-IX-alpha, Hordatin A and N-demethyl mifepristone in the CsA-induced hypoimmunity lung injury model.

Conclusion: MXSGD can restore gut and lung microbiota diversity and serum metabolite changes to inhibit inflammation, ameliorate CsA-induced hypoimmunity lung injury.

Keywords: CSA; MXSGD; flora metabolism; hypoimmunity; lung injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter*
  • Animals
  • Cyclosporine
  • Drugs, Chinese Herbal*
  • Immunologic Deficiency Syndromes*
  • Lung Injury* / chemically induced
  • Lung Injury* / drug therapy
  • Mice
  • RNA, Ribosomal, 16S / genetics

Substances

  • Cyclosporine
  • maxingshigan
  • RNA, Ribosomal, 16S
  • Drugs, Chinese Herbal

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by project of Changsha Outstanding Innovative Youth Training program (kh2307003); Hunan Natural Science Foundation (No:2021JJ30508); Project of Hunan provincial Health Commission (No: D202301067791); Provincial Discipline Construction Project of Hunan University of Traditional Chinese Medicine (Integrated Traditional Chinese and Western Medicine) Innovative topics of university graduates in 2022 (No:2022CX184) The Open Foundation of the Hunan Key Laboratory of Pathogenic Biology of Integrated Traditional Chinese and Western Medicine (No. 2022KFJJ05).