Mass Spectrometry Imaging of Organoids to Improve Preclinical Research

Cristina Zivko; Tae-Hun Hahm; Cay Tressler; Dalton Brown; Kristine Glunde; Vasiliki Mahairaki

doi:10.1002/adhm.202302499

Mass Spectrometry Imaging of Organoids to Improve Preclinical Research

Adv Healthc Mater. 2024 Jan 21:e2302499. doi: 10.1002/adhm.202302499. Online ahead of print.

Authors

Cristina Zivko^{1

2}, Tae-Hun Hahm³, Cay Tressler³, Dalton Brown³, Kristine Glunde^{3

4

5}, Vasiliki Mahairaki^{1

2}

Affiliations

¹ Department of Genetic Medicine, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.
² The Richman Family Precision Medicine Center of Excellence in Alzheimer's Disease, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.
³ Russell H. Morgan Department of Radiology and Radiological Science, Division of Cancer Imaging Research, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.
⁴ The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, MD, 21231, USA.
⁵ Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.

PMID: 38247228
DOI: 10.1002/adhm.202302499

Abstract

Preclinical models are essential research tools before novel therapeutic or diagnostic methods can be applied to humans. These range from in vitro cell monocultures to vastly more complex animal models, but clinical translation to humans often fails to deliver significant results. Three-dimensional (3D) organoid systems are being increasingly studied to establish physiologically relevant in vitro platforms in a trade-off between the complexity of the research question and the complexity of practical experimental setups. The sensitivity and precision of analytical tools are yet another limiting factors in what can be investigated, and mass spectrometry (MS) is one of the most powerful analytical techniques available to the scientific community. Its innovative use to spatially resolve biological samples has opened many research avenues in the field of MS imaging (MSI). Here, this work aims to explore the current scientific landscape in the application of MSI on organoids, with an emphasis on their combined potential to facilitate and improve preclinical studies.

Keywords: MALDI; iPSCs; mass spectometry; organoids; precision medicine.

Publication types

Review

Abstract

Publication types

Grants and funding