Distinct antiviral activities of IFNφ1 and IFNφ4 in zebrafish

Kangyong Chen; Jiayin Tian; Yanjie Shi; Teng Xie; Wenji Huang; Zhao Jia; Yanwei Zhang; Gaoliang Yuan; Hui Yan; Junya Wang; Jun Zou

doi:10.1016/j.fsi.2024.109396

Distinct antiviral activities of IFNφ1 and IFNφ4 in zebrafish

Fish Shellfish Immunol. 2024 Mar:146:109396. doi: 10.1016/j.fsi.2024.109396. Epub 2024 Jan 18.

Authors

Kangyong Chen¹, Jiayin Tian¹, Yanjie Shi¹, Teng Xie¹, Wenji Huang¹, Zhao Jia¹, Yanwei Zhang¹, Gaoliang Yuan¹, Hui Yan¹, Junya Wang¹, Jun Zou²

Affiliations

¹ Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China.
² Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266200, China. Electronic address: jzou@shou.edu.cn.

PMID: 38244820
DOI: 10.1016/j.fsi.2024.109396

Abstract

Interferons (IFNs) are a group of secreted cytokines that play a crucial role in antiviral immunity. Type I IFNs display functional disparities. In teleosts, type I IFNs are categorized into two subgroups containing one or two pairs of disulfide bonds. However, their functional differences have not been fully elucidated. In this study, we comparatively characterized the antiviral activities of zebrafish IFNφ1 and IFNφ4 belonging to the group I type I IFNs. It was found that ifnφ1 and ifnφ4 were differentially modulated during viral infection. Although both IFNφ1 and IFNφ4 activated JAK-STAT signaling pathway via CRFB1/CRFB5 receptor complex, IFNφ4 was less potent in inducing phosphorylation of STAT1a, STAT1b and STAT2 and the expression of antiviral genes than IFNφ1, thereby conferring weaker antiviral resistance of target cells. Taken together, our results provide insights into the functional divergence of type I IFNs in lower vertebrates.

Keywords: Antiviral response; Interferon; Virus; Zebrafish.

MeSH terms

Animals
Cytokines / genetics
Interferon Type I* / genetics
Interferons / metabolism
Perciformes* / metabolism
Phosphorylation
Zebrafish
Zebrafish Proteins / genetics
Zebrafish Proteins / metabolism

Substances

Zebrafish Proteins
Interferons
Cytokines
Interferon Type I