High-throughput CRISPR technology: a novel horizon for solid organ transplantation

Xiaohan Li; Zhang Chen; Weicong Ye; Jizhang Yu; Xi Zhang; Yuan Li; Yuqing Niu; Shuan Ran; Song Wang; Zilong Luo; Jiulu Zhao; Yanglin Hao; Junjie Zong; Chengkun Xia; Jiahong Xia; Jie Wu

doi:10.3389/fimmu.2023.1295523

High-throughput CRISPR technology: a novel horizon for solid organ transplantation

Front Immunol. 2024 Jan 4:14:1295523. doi: 10.3389/fimmu.2023.1295523. eCollection 2023.

Authors

Xiaohan Li^#^{1

2}, Zhang Chen^#^{1

2}, Weicong Ye^#^{1

2}, Jizhang Yu^{1

2}, Xi Zhang^{1

2}, Yuan Li^{1

2}, Yuqing Niu^{1

2}, Shuan Ran^{1

2}, Song Wang^{1

2}, Zilong Luo^{1

2}, Jiulu Zhao^{1

2}, Yanglin Hao^{1

2}, Junjie Zong^{1

2}, Chengkun Xia³, Jiahong Xia^{1

2

4}, Jie Wu^{1

2

4}

Affiliations

¹ Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
² Center for Translational Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
³ Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
⁴ Key Laboratory of Organ Transplantation, Ministry of Education, National Health Commission (NHC) Key Laboratory of Organ Transplantation, Key Laboratory of Organ Transplantation, Chinese Academy of Medical Sciences, Wuhan, China.

^# Contributed equally.

Abstract

Organ transplantation is the gold standard therapy for end-stage organ failure. However, the shortage of available grafts and long-term graft dysfunction remain the primary barriers to organ transplantation. Exploring approaches to solve these issues is urgent, and CRISPR/Cas9-based transcriptome editing provides one potential solution. Furthermore, combining CRISPR/Cas9-based gene editing with an ex vivo organ perfusion system would enable pre-implantation transcriptome editing of grafts. How to determine effective intervention targets becomes a new problem. Fortunately, the advent of high-throughput CRISPR screening has dramatically accelerated the effective targets. This review summarizes the current advancements, utilization, and workflow of CRISPR screening in various immune and non-immune cells. It also discusses the ongoing applications of CRISPR/Cas-based gene editing in transplantation and the prospective applications of CRISPR screening in solid organ transplantation.

Keywords: CRISPR screening; CRISPR/Cas9; high-throughput; solid organ transplantation; transcriptome editing.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems*
Gene Editing*

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by Fundamental Research Funds for the Central Universities [2021GCRC037 to JX, and YCJJ20230232 to SR], the National Natural Science Foundation of China [82241217 and 82071803 to JW, 82271811, 82170504, and 81730015 to JX], the project funded by China Postdoctoral Science Foundation [2021M691155 to JY, and 2023M741285 to XZ], and the Science foundation of union hospital [2021xhyn096 to JY].