New discovery of extremely high adsorption of environmental DNA on cuttlefish bone pyrolysis derivative via large pore structure and carbon film

Lin Shi; Di Zhang; Mingyi Yang; Fangfang Li; Jinfeng Zhao; Zhaohui He; Yangwei Bai

doi:10.1016/j.wasman.2024.01.016

New discovery of extremely high adsorption of environmental DNA on cuttlefish bone pyrolysis derivative via large pore structure and carbon film

Waste Manag. 2024 Mar 1:175:286-293. doi: 10.1016/j.wasman.2024.01.016. Epub 2024 Jan 17.

Authors

Lin Shi¹, Di Zhang², Mingyi Yang³, Fangfang Li⁴, Jinfeng Zhao⁵, Zhaohui He⁶, Yangwei Bai⁷

Affiliations

¹ Shandong Provincial Key Laboratory of Water and Soil Conservation and Environmental Protection, College of Resources and Environment, Linyi University, Linyi, 276005, China. Electronic address: shilin@lyu.edu.cn.
² Shandong Provincial Key Laboratory of Water and Soil Conservation and Environmental Protection, College of Resources and Environment, Linyi University, Linyi, 276005, China; Yunnan Provincial Key Laboratory of Soil Carbon Sequestration and Pollution Control, Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, China. Electronic address: zhangdi2002113@lyu.edu.cn.
³ Yunnan Provincial Key Laboratory of Soil Carbon Sequestration and Pollution Control, Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, China. Electronic address: 970462884@qq.com.
⁴ Yunnan Provincial Key Laboratory of Soil Carbon Sequestration and Pollution Control, Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, China. Electronic address: FFLi@kust.edu.cn.
⁵ Shandong Provincial Key Laboratory of Water and Soil Conservation and Environmental Protection, College of Resources and Environment, Linyi University, Linyi, 276005, China. Electronic address: zhaojinfeng@lyu.edu.cn.
⁶ Yunnan Provincial Key Laboratory of Soil Carbon Sequestration and Pollution Control, Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, China. Electronic address: 58557760@qq.com.
⁷ Chinese Research Academy of Environmental Sciences, Beijing, 100012, China. Electronic address: baiyw@craes.org.cn.

PMID: 38237404
DOI: 10.1016/j.wasman.2024.01.016

Abstract

Environmental DNA (eDNA) carrying antibiotic resistance gene (ARG) has attracted a great deal of attention because of its threat to the ecology and human health. Traditional porous adsorbents, such as microporous biochar and natural mineral, are low-effective in removing eDNA from sewage. This study used cuttlefish-bone (CB), a fishery waste, as an anticipated material to adsorb a model compound of eDNA from herring sperm (hsDNA). An interesting result was firstly observed that extremely high DNA adsorption on cuttlefish-bone pyrolysis derivative (CCB) was up to 88.7 mg/g, 3-10 folds higher than that of most other adsorbents in the existing literatures, which was attributed to the carbon film and large pores. To achieve an adsorption rate of 75 %, hsDNA adsorption took 96 h on CB but only 24 h on CCB, which was attributed to the fluent channel of CCB. The ligand exchange, Ca²⁺ bridge and π-π interaction were identified as dominated adsorption mechanisms, based on FTIR and phosphate competition experiments. This study exploited a high-efficient, environmentally friendly, and low-cost adsorbent for treating ARG-contaminated soil and water.

Keywords: Biological calcium carbonate; High-efficient adsorption; Porous matrix; eDNA.

MeSH terms

Adsorption
Anti-Bacterial Agents
Carbon / chemistry
Charcoal / chemistry
DNA, Environmental*
Humans
Kinetics
Male
Pyrolysis
Semen / chemistry
Water Pollutants, Chemical* / analysis

Substances

Carbon
DNA, Environmental
Charcoal
Anti-Bacterial Agents
Water Pollutants, Chemical