Effect of Lactobacillus plantarum ZFM4 in Helicobacter pylori-infected C57BL/6 mice: prevention is better than cure

Ying-Ying Yu; Ling-Yan Wu; Xue Sun; Qing Gu; Qing-Qing Zhou

doi:10.3389/fcimb.2023.1320819

Effect of Lactobacillus plantarum ZFM4 in Helicobacter pylori-infected C57BL/6 mice: prevention is better than cure

Front Cell Infect Microbiol. 2024 Jan 3:13:1320819. doi: 10.3389/fcimb.2023.1320819. eCollection 2023.

Authors

Ying-Ying Yu^{1

2}, Ling-Yan Wu¹, Xue Sun¹, Qing Gu³, Qing-Qing Zhou³

Affiliations

¹ Department of general practice, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
² The Key Laboratory of Intelligent Preventive Medicine of Zhejiang Province, Hangzhou, Zhejiang, China.
³ Key Laboratory for Food Microbial Technology of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang, China.

Abstract

Objectives: This study was performed to explore the preventive and therapeutic effects of Lactobacillus plantarum ZFM4 on H. pylori infections of the stomach tissue in C57BL/6 mice.

Methods: In this study, 40 specific-pathogen-free female C57BL/6 mice were randomly divided into five groups, namely, the control, ZFM4 pretreatment) ZFM4 pretreatment before H. pylori infected), model (H. pylori infected), triple therapy (H. pylori infected and treated with triple therapy), and ZFM4 treatment groups (H. pylori infected and treated with ZFM4). The preventive and therapeutic effects of Lactobacillus plantarum ZFM4 were evaluated in H. pylori-infected C57BL/6 mice by assessing gastric tissue morphology, inflammatory cytokine levels, microbial composition, and microbial diversity.

Results: Lactobacillus plantarum ZFM4 was able to survive in low gastric pH and play a role in preventing H. pylori infection. This was evident from a reduction in both, the gastric inflammatory response and expression of inflammatory factors caused by H. pylori infection. Lactobacillus plantarum ZFM4 could also inhibit the growth of H. pylori via its beneficial impact on the gastric microbiota.

Conclusion: Our findings suggest that Lactobacillus plantarum ZFM4 offers superior preventive effects against H. pylori infections when used alone. However, the therapeutic effect on established infections is weaker. Further clinical trials are needed to confirm the specific dosage, duration, and other aspects of administration.

Keywords: 16s rRNA sequencing; Helicobacter pylori; Lactobacillus plantarum ZFM4; prevention; probiotics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Female
Gastric Mucosa
Helicobacter Infections* / drug therapy
Helicobacter Infections* / prevention & control
Helicobacter pylori*
Lactobacillus plantarum* / physiology
Mice
Mice, Inbred C57BL
Probiotics* / therapeutic use
Stomach

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the Zhejiang Provincial Natural Science Foundation of China (LGF19H030016) and the Medical and Health Technology Projects of Zhejiang Province (2018KY413 and 2022KY815).