Dataset describing the amino acid catabolism of Thermoanaerobacter pseudethanolicus

Johann Orlygsson; Sean Michael Scully

doi:10.1016/j.dib.2023.110017

Dataset describing the amino acid catabolism of Thermoanaerobacter pseudethanolicus

Data Brief. 2023 Dec 27:52:110017. doi: 10.1016/j.dib.2023.110017. eCollection 2024 Feb.

Authors

Johann Orlygsson¹, Sean Michael Scully¹

Affiliation

¹ University of Akureyri, Faculty of Natural Resource Sciences, Borgir 2 v/ Norðurslóð, 600 Akureyri, Iceland.

Abstract

The dataset depicts the catabolism of branched-chain amino acids by Thermoanaerobacter pseudethanolicus in the presence of thiosulfate under different culture conditions. The results reveal that the strain can degrade all three branched-chain amino acids resulting in the production of their corresponding branched-chain fatty acids and branched-chain alcohols with the fatty acids always being the dominant product. The highest amounts of 2-methyl-1-butanol from isoleucine were at pH 6.5, liquid-gas ratio of 0.98, and at 20 mM thiosulfate concentration. A kinetic experiment of the branched-chain amino acids was done in the presence of thiosulfate as are data on selected enzyme activities related to alcohols and aldehydes. Finally, an NMR study using ¹³C1 methyl-1-butyrate during the degradation of leucine in the presence of thiosulfate was done to prove that the ¹³C1-methyl-1-butanol was indeed from its corresponding fatty acid.

Keywords: Alcohols, and alcohols; Anaerobic; Branched-chain amino acids; Thermophilic; Thiosulfate.