Overexpression and knockdown of cotton GhdadD gene reveals its drought and salt stress tolerance role

Yuanyuan Zhang; Jie Zheng; Shiraku Margaret Linyerera; Richard Odongo Magwanga; Yuqing Hou; Yuhong Wang; Yanchao Xu; Aziz Khan; Shuxun Yu; Zhongli Zhou; Fang Liu; Xiaoyan Cai

doi:10.1016/j.isci.2023.108664

Overexpression and knockdown of cotton GhdadD gene reveals its drought and salt stress tolerance role

iScience. 2023 Dec 7;27(1):108664. doi: 10.1016/j.isci.2023.108664. eCollection 2024 Jan 19.

Authors

Yuanyuan Zhang^{1

2}, Jie Zheng², Shiraku Margaret Linyerera², Richard Odongo Magwanga², Yuqing Hou², Yuhong Wang², Yanchao Xu², Aziz Khan³, Shuxun Yu², Zhongli Zhou², Fang Liu^{2

4}, Xiaoyan Cai^{1

2}

Affiliations

¹ National Nanfan Research Institute (Sanya), Chinese Academy of Agriculture Sciences, Sanya 572025, China.
² National Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Institute of Cotton Research, Chinese Academy of Agricultural Sciences (ICR, CAAS), Anyang, Henan 455000, China.
³ State Key Laboratory of Grassland Agro-ecosystems, College of Ecology, Lanzhou University, Lanzhou 730000, China.
⁴ School of Agricultural Sciences, Zhengzhou University, Zhengzhou, China.

Abstract

The 5'-deoxyadenosine deaminase (DADD), a member of the amidohydrolase family regulates biological purine metabolism. In this study, bioinformatic analysis, overexpression and knockdown of GhdadD gene were detected to identify its potential role in drought and salt stress tolerance. The results revealed that GhdadD was induced by ABA, Auxin, MBS and light responsive elements. In transgenic Arabidopsis, seed germination rate and root length were increased under drought or salt stress. GhdadD overexpressed seedlings resulted in higher plant height, less leaf damage and lower ion permeability. The expression of osmotic stress and ABA-responsive genes were up regulated. While in GhdadD-silenced cotton seedlings, CAT, SOD activity and soluble sugar content were reduced, MDA content was increased, and the stoma opening was depressed under drought or salt stress. Some osmics stress marker genes were also up regulated. These data indicating that GhdadD enhanced plant resistance to drought and salt stress through ABA pathways.

Keywords: Biochemistry; Genetics; Plant physiology.