Monitoring of optical properties of tumors during laser plasmon photothermal therapy

Vadim D Genin; Alla B Bucharskaya; Mikhail Yu Kirillin; Daria A Kurakina; Nikita A Navolokin; Georgy S Terentyuk; Boris N Khlebtsov; Nikolai G Khlebtsov; Galina N Maslyakova; Valery V Tuchin; Elina A Genina

doi:10.1002/jbio.202300322

Monitoring of optical properties of tumors during laser plasmon photothermal therapy

J Biophotonics. 2024 Apr;17(4):e202300322. doi: 10.1002/jbio.202300322. Epub 2024 Jan 14.

Authors

Vadim D Genin^{1

2}, Alla B Bucharskaya^{2

3}, Mikhail Yu Kirillin^{4

5}, Daria A Kurakina⁴, Nikita A Navolokin³, Georgy S Terentyuk³, Boris N Khlebtsov⁶, Nikolai G Khlebtsov^{1

6}, Galina N Maslyakova³, Valery V Tuchin^{1

2

7}, Elina A Genina^{1

2}

Affiliations

¹ Optics and Biophotonics Department, Saratov State University, Saratov, Russia.
² Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, Tomsk, Russia.
³ Core Facility of Experimental Oncology, Saratov State Medical University named after V. I. Razumovsky, Saratov, Russia.
⁴ Biophotonics Laboratory, Institute of Applied Physics Russian Academy of Sciences, Nizhny Novgorod, Russia.
⁵ Applied Mathematics Department, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia.
⁶ Laboratory of Nanobiotechnology, Institute of Biochemistry and Physiology of Plants and Microorganisms, Federal Research Centre "Saratov Scientific Centre of the Russian Academy of Sciences" (IBPPM RAS), Saratov, Russia.
⁷ Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control, Federal Research Centre "Saratov Scientific Centre of the Russian Academy of Sciences", Saratov, Russia.

PMID: 38221797
DOI: 10.1002/jbio.202300322

Abstract

We studied grafted tumors obtained by subcutaneous implantation of kidney cancer cells into male white rats. Gold nanorods with a plasmon resonance of about 800 nm were injected intratumorally for photothermal heating. Experimental irradiation of tumors was carried out percutaneously using a near-infrared diode laser. Changes in the optical properties of the studied tissues in the spectral range 350-2200 nm under plasmonic photothermal therapy (PPT) were studied. Analysis of the observed changes in the absorption bands of water and hemoglobin made it possible to estimate the depth of thermal damage to the tumor. A significant decrease in absorption peaks was observed in the spectrum of the upper peripheral part and especially the tumor capsule. The obtained changes in the optical properties of tissues under laser irradiation can be used to optimize laboratory and clinical PPT procedures.

Keywords: gold nanorods; kidney cancer; optical properties; plasmonic photothermal therapy.

MeSH terms

Animals
Gold / therapeutic use
Laser Therapy*
Lasers, Semiconductor
Male
Nanotubes*
Neoplasms*
Photothermal Therapy
Rats

Substances

Gold

Abstract

MeSH terms

Substances

Grants and funding