Specific ions effect on aggregation behaviors and structural changes of amyloid fibrils from rice glutelin

Ting Li; Dong Wang; Xinxia Zhang; Zhengxing Chen; Li Wang

doi:10.1016/j.foodchem.2023.138351

Specific ions effect on aggregation behaviors and structural changes of amyloid fibrils from rice glutelin

Food Chem. 2024 May 30:441:138351. doi: 10.1016/j.foodchem.2023.138351. Epub 2024 Jan 5.

Authors

Ting Li¹, Dong Wang¹, Xinxia Zhang¹, Zhengxing Chen², Li Wang³

Affiliations

¹ Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; Jiangsu Provincial Engineering Research Center for Bioactive Product Processing, Jiangnan University, Lihu Road 1800, Wuxi 214122, China.
² Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; Jiangsu Provincial Engineering Research Center for Bioactive Product Processing, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; State Key Laboratory of Food Science and Resources, Jiangnan University, Lihu Road 1800, Wuxi 214122, China.
³ Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; Jiangsu Provincial Engineering Research Center for Bioactive Product Processing, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; State Key Laboratory of Food Science and Resources, Jiangnan University, Lihu Road 1800, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, China. Electronic address: wangli0318@jiangnan.edu.cn.

PMID: 38218147
DOI: 10.1016/j.foodchem.2023.138351

Abstract

Metal ions have been considered as an important factor on fibrils assembly. Herein, a comprehensive analysis of specific ions effect on fibril formation and structural changes was investigated. The addition of ions (except Zn²⁺) accelerated the aggregation kinetics of rice glutelin fibrils (RGFs) from 0.93 to 1.28-2.19 h^-1. In addition, the fibrillization rate followed the order of NH₄⁺ > Li⁺ > Na⁺ > K⁺ > Cu²⁺ > Mg²⁺ > Ca²⁺ > Zn²⁺. The highest yield and length of fibrils were observed with Ca²⁺, probably due to the ionic bridging effect and hydrated capacity of Ca²⁺. However, Cu²⁺ reduced the fibrils yield, which was attributable to the fact that Cu²⁺ disrupted β-sheet structure and inhibited the transition of monomer to fibrils. The polymorphism of fibrils was observed with different salts, and the light metals presented a superior effect on fibrils formation than heavy metals. Overall, this work will provide a further information into how to tune the structure of RGFs using various ions.

Keywords: Aggregation behavior; Rice glutelin fibrils; Specific ions effect; Structural changes.

MeSH terms

Amyloid beta-Peptides / chemistry
Amyloid* / chemistry
Glutens
Ions / chemistry
Oryza*

Substances

Amyloid
Amyloid beta-Peptides
Glutens
Ions