Diet Quality and Epigenetic Aging in the Women's Health Initiative

Lindsay M Reynolds; Denise K Houston; Meghan B Skiba; Eric A Whitsel; James D Stewart; Yun Li; Anthony S Zannas; Themistocles L Assimes; Steve Horvath; Parveen Bhatti; Andrea A Baccarelli; Janet A Tooze; Mara Z Vitolins

doi:10.1016/j.jand.2024.01.002

Diet Quality and Epigenetic Aging in the Women's Health Initiative

J Acad Nutr Diet. 2024 Jan 11:S2212-2672(24)00002-9. doi: 10.1016/j.jand.2024.01.002. Online ahead of print.

Authors

Affiliations

¹ Division of Public Health Sciences, Wake Forest University School of Medicine, Winston-Salem, North Carolina. Electronic address: lireynol@wakehealth.edu.
² Department of Internal Medicine, Wake Forest University School of Medicine, Winston-Salem, North Carolina.
³ Division of Biobehavioral Health Science, University of Arizona Cancer Center, University of Arizona, Tucson, Arizona.
⁴ Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina.
⁵ Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina; Department of Medicine, School of Medicine, University of North Carolina, Chapel Hill, North Carolina.
⁶ Department of Genetics, School of Medicine, University of North Carolina, Chapel Hill, North Carolina; Department of Biostatistics, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina.
⁷ Department of Psychiatry, University of North Carolina, Chapel Hill, North Carolina.
⁸ Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Palo Alto, California.
⁹ Department of Human Genetics, University of California Los Angeles, Los Angeles, California; Altos Labs, San Diego, California.
¹⁰ Cancer Control Research, BC Cancer Research Institute, Vancouver, British Columbia, Canada; School of Population and Public Health, University of British Columbia, Vancouver, British Columbia, Canada.
¹¹ Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, New York.
¹² Division of Public Health Sciences, Wake Forest University School of Medicine, Winston-Salem, North Carolina.

PMID: 38215906
DOI: 10.1016/j.jand.2024.01.002

Abstract

Background: Higher diet quality scores are associated with a lower risk for many chronic diseases and all-cause mortality; however, it is unclear if diet quality is associated with aging biology.

Objective: This study aimed to examine the association between diet quality and a measure of biological aging known as epigenetic aging.

Design: A cross-sectional data analysis was used to examine the association between three diet quality scores based on self-reported food frequency questionnaire data and five measures of epigenetic aging based on DNA methylation (DNAm) data from peripheral blood.

Participants/setting: This study included 4,500 postmenopausal women recruited from multiple sites across the United States (1993-98), aged 50 to 79 years, with food frequency questionnaire and DNAm data available from the Women's Health Initiative baseline visit.

Main outcome measures: Five established epigenetic aging measures were generated from HumanMethylation450 Beadchip DNAm data, including AgeAccelHannum, AgeAccelHorvath, AgeAccelPheno, AgeAccelGrim, and DunedinPACE.

Statistical analyses performed: Linear mixed models were used to test for associations between three diet quality scores (Healthy Eating Index, Dietary Approaches to Stop Hypertension, and alternate Mediterranean diet scores) and epigenetic aging measures, adjusted for age, race and ethnicity, education, tobacco smoking, physical activity, Women's Health Initiative substudy from which DNAm data were obtained, and DNAm-based estimates of leukocyte proportions.

Results: Healthy Eating Index, Dietary Approaches to Stop Hypertension, and alternate Mediterranean diet scores were all inversely associated with AgeAccelPheno, AgeAccelGrim, and DunedinPACE (P < 0.05), with the largest effects with DunedinPACE. A one standard deviation increment in diet quality scores was associated with a decrement (β ± SE) in DunedinPACE z score of -0.097 ± 0.014 (P = 9.70 x 10^-13) for Healthy Eating Index, -0.107 ± 0.014 (P = 1.53 x 10^-14) for Dietary Approaches to Stop Hypertension, and -0.068 ± 0.013 (P = 2.31 x 10^-07) for the alternate Mediterranean diet.

Conclusions: In postmenopausal women, diet quality scores were inversely associated with DNAm-based measures of biological aging, particularly DunedinPACE.

Keywords: Aging; DNA methylation; Diet quality; Women; epigenetic Aging.

Grants and funding

P30 AG021332/AG/NIA NIH HHS/United States