Hydrolytic degradation of methoxychlor by immobilized cellulase on LDHs@Fe3O4 nanocomposites

Nazar Iqbal; Guangda Mu; Mengyang Dong; Yuxiang Yang; Yan Huang; Hongming Yuan; Xiangnong Liu; Irum Batool; Riccardo Carlini

doi:10.2166/wst.2023.399

Hydrolytic degradation of methoxychlor by immobilized cellulase on LDHs@Fe₃O₄ nanocomposites

Water Sci Technol. 2024 Jan;89(1):170-186. doi: 10.2166/wst.2023.399.

Authors

Nazar Iqbal¹, Guangda Mu², Mengyang Dong³, Yuxiang Yang³, Yan Huang³, Hongming Yuan⁴, Xiangnong Liu⁵, Irum Batool³, Riccardo Carlini⁶

Affiliations

¹ School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China; Nazar lqbal and Guangda Mu contributed equally to this work E-mail: yxyang@ecust.edu.cn.
² School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China; Nazar lqbal and Guangda Mu contributed equally to this work.
³ School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
⁴ State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University, Changchun 130012, China.
⁵ Analysis Test Center, Yangzhou University, Yangzhou 225009, China.
⁶ Chemistry and Material Chemistry Department, LAS Klee-Barabino, 16146 Genova, Italy.

PMID: 38214993
DOI: 10.2166/wst.2023.399

Abstract

In this study, we synthesized Fe₃O₄ using the co-precipitation method and then prepared magnetic carrier LDHs@Fe₃O₄ by immobilizing layered double hydroxide on Fe₃O₄ by in situ growth method. Cellulase was immobilized on this magnetic carrier by using glutaraldehyde as a coupling agent, which can be used for degrading Methoxychlor (MXC). The results demonstrated the maximum MXC removal efficiency of 73.4% at 45 °C and pH = 6.0 with excellent reusability. Through kinetic analysis, it was found that the degradation reaction conforms to the Langmuir-Hinshelwood model and is a first-order reaction. Finally, according to the EPR analysis, the active radicals in the system were found to be OH· and the degradation mechanism was proposed in combination with LC-MS. This study provides a feasible method for degrading organochlorine pesticides, which can be used for groundwater purification.

MeSH terms

Cellulase*
Hydroxides*
Kinetics
Methoxychlor
Nanocomposites*

Substances

Methoxychlor
Cellulase
hydroxide ion
Hydroxides