A proteomic insight reveals the role of food-associated Lactiplantibacillus plantarum C9O4 in reverting intestinal inflammation

Maria Concetta Cufaro; Roberta Prete; Federica Di Marco; Giusi Sabatini; Aldo Corsetti; Natalia Garcia Gonzalez; Piero Del Boccio; Natalia Battista

doi:10.1016/j.isci.2023.108481

A proteomic insight reveals the role of food-associated Lactiplantibacillus plantarum C9O4 in reverting intestinal inflammation

iScience. 2023 Nov 17;26(12):108481. doi: 10.1016/j.isci.2023.108481. eCollection 2023 Dec 15.

Authors

Maria Concetta Cufaro^{1

2}, Roberta Prete³, Federica Di Marco^{1

2}, Giusi Sabatini³, Aldo Corsetti³, Natalia Garcia Gonzalez³, Piero Del Boccio^{1

4}, Natalia Battista³

Affiliations

¹ Center for Advanced Studies and Technology (CAST), 66100 Chieti, Italy.
² Department of Innovative Technologies in Medicine & Dentistry, "G. d'Annunzio" University of Chieti-Pescara, 66100 Chieti, Italy.
³ Department of Bioscience for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, Italy.
⁴ Department of Pharmacy, "G. d'Annunzio" University of Chieti-Pescara, 66100 Chieti, Italy.

Abstract

Nowadays, Western diets and lifestyle lead to an increasing occurrence of chronic gut inflammation that represents an emerging health concern with still a lack of successful therapies. Fermented foods, and their associated lactic acid bacteria, have recently regained popularity for their probiotic potential including the maintenance of gut homeostasis by modulating the immune and inflammatory response. Our study aims to investigate the crosstalk between the food-borne strain Lactiplantibacillus plantarum C9O4 and intestinal epithelial cells in an in vitro inflammation model. Cytokines profile shows the ability of C9O4 to significantly reduce levels of IL-2, IL-5, IL-6, and IFN-γ. Proteomic functional analysis reveals an immunoregulatory role of C9O4, able to revert the detrimental effects of IFN-γ through the JAK/STAT pathway in inflamed intestinal cells. These results suggest a promising therapeutic role of fermented food-associated microbes for the management of gastrointestinal inflammatory diseases. Data are available via ProteomeXchange with identifier PXD042175.

Keywords: Food chemistry; Gastroenterology; Molecular biology.