The metabolite profiles of two plant growth promoting cyanobacteria-Anabaena laxa and Calothrix elenkinii, which serve as promising biofertilizers, and biocontrol agents were generated to investigate their agriculturally beneficial activities. Preliminary biochemical analyses, in terms of total chlorophyll, total proteins, and IAA were highest at 14 days after inoculation (DAI). In A. laxa 20-25% higher values of reducing sugars, than C. elenkinii at both 14 and 21 DAI were recorded. Carbon and nitrogen assimilating enzyme activities-phosphoenol pyruvate carboxylase (PEPC), carbonic anhydrase (CA), and glutamine synthetase (GS) were highest at 14 DAI, albeit, nitrate reductase (NR) activity was higher by 0.73-0.84-fold at 21 DAI. Untargeted GC-MS (Gas chromatography-Mass spectrometric) analysis of metabolite profiles of 21d-old cyanobacterial cultures and characterization using NIST mass spectral library illustrated that A. laxa recorded highest number of metabolite hits in three chemical classes namely amino acid and peptides, nucleotides, nucleosides and analogues, besides other organic compounds. Based on the pathway analysis of identified metabolites, both A. laxa, and C. elenkinii were enriched in metabolites involved in aminoacyl-tRNA biosynthesis, and amino acid metabolism pathways, particularly lactose and glutamic acid, which are important players in plant-microbe interactions. Correlation-based metabolite network illustrated distinct and significant differences in the metabolic machinery of A. laxa and C. elenkinii, highlighting their novel identity and enrichment in C-N rich metabolites, as factors underlying their plant growth and soil fertility enhancing attributes, which make them valuable as inoculants.
Supplementary information: The online version contains supplementary material available at 10.1007/s13205-023-03902-7.
Keywords: Amino acids; Cyanobacteria; Enzymes; Metabolites; Sugars.
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