The sound of silent RNA in tuberculosis and the lncRNA role on infection

Eduardo Fukutani Rocha; Caian Leal Vinhaes; Mariana Araújo-Pereira; Tiago Feitosa Mota; Akshay N Gupte; Nathella Pavan Kumar; Maria Belen Arriaga; Timothy R Sterling; Subash Babu; Sanjay Gaikwad; Rajesh Karyakarte; Vidya Mave; Vandana Kulkarni; Mandar Paradkar; Vijay Viswanathan; Hardy Kornfeld; Amita Gupta; Bruno Bezerril Andrade; Artur Trancoso Lopo de Queiroz; RePORT Brazil; RePORT India Consortia

doi:10.1016/j.isci.2023.108662

The sound of silent RNA in tuberculosis and the lncRNA role on infection

iScience. 2023 Dec 8;27(1):108662. doi: 10.1016/j.isci.2023.108662. eCollection 2024 Jan 19.

Authors

Eduardo Fukutani Rocha^{1

2}, Caian Leal Vinhaes^{3

2

4}, Mariana Araújo-Pereira^{3

2

4

5}, Tiago Feitosa Mota^{1

2}, Akshay N Gupte⁶, Nathella Pavan Kumar⁷, Maria Belen Arriaga^{3

2}, Timothy R Sterling⁸, Subash Babu⁹, Sanjay Gaikwad¹⁰, Rajesh Karyakarte¹¹, Vidya Mave^{12

13}, Vandana Kulkarni^{12

13}, Mandar Paradkar^{12

13}, Vijay Viswanathan¹⁴, Hardy Kornfeld^{15

16}, Amita Gupta¹², Bruno Bezerril Andrade^{3

2

4

5}, Artur Trancoso Lopo de Queiroz^{1

3

2}; RePORT Brazil; RePORT India Consortia

Affiliations

¹ Centro de Integração de Dados e Conhecimentos para Saúde, Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil.
² Multinational Organization Network Sponsoring Translational and Epidemiological Research (MONSTER) Initiative, Salvador, Brazil.
³ Laboratório de Inflamação e Biomarcadores, Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil.
⁴ Escola Bahiana de Medicina e Saúde Pública (EBMSP), Salvador 40290-150, Brazil.
⁵ Faculdade de Tecnologia e Ciências, Instituto de Pesquisa Clínica e Translacional, Salvador, Brazil.
⁶ Boston University School of Public Health, Boston, MA USA.
⁷ ICMR-National Institute for Research in Tuberculosis, Chennai, India.
⁸ Division of Infectious Diseases, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN USA.
⁹ National Institutes of Health- NIRT - International Center for Excellence in Research, Chennai, India.
¹⁰ Department of Pulmonary Medicine, Byramjee-Jeejeebhoy Government Medical College and Sassoon General Hospitals, Pune, India.
¹¹ Department of Microbiology, Byramjee-Jeejeebhoy Government Medical College and Sassoon General Hospitals, Pune, India.
¹² Byramjee-Jeejeebhoy Government Medical College-Johns Hopkins University Clinical Research Site, Pune, India.
¹³ Johns Hopkins Center for Infectious Diseases in India, Pune, India.
¹⁴ Prof. M. Viswanathan Diabetes Research Centre, Chennai, India.
¹⁵ Department of Medicine, University of Massachusetts Medical School, Worcester, MA USA.
¹⁶ UMass Chan Medical School, Worcester, MA USA.

Abstract

Tuberculosis (TB) is one of the leading causes of death worldwide, and Diabetes Mellitus is one of the major comorbidities (TB/DM) associated with the disease. A total of 103 differentially expressed ncRNAs have been identified in the TB and TB/DM comparisons. A machine learning algorithm was employed to identify the most informative lncRNAs: ADM-DT, LINC02009, LINC02471, SOX2-OT, and GK-AS1. These lncRNAs presented substantial accuracy in classifying TB from HC (AUCs >0.85) and TB/DM from HC (AUCs >0.90) in the other three countries. Genes with significant correlations with the five lncRNAs enriched common pathways in Brazil and India for both TB and TB/DM. This suggests that lncRNAs play an important role in the regulation of genes related to the TB immune response.

Keywords: Health sciences; Infection control in health technology.

Abstract

Grants and funding