Grafting chlorogenic acid enhanced the antioxidant activity of curdlan oligosaccharides and modulated gut microbiota

Huan Li; Wenjiang He; Saiqing Xu; Rongrong Wang; Shuai Ge; Haishan Xu; Yang Shan; Shenghua Ding

doi:10.1016/j.fochx.2023.101075

Grafting chlorogenic acid enhanced the antioxidant activity of curdlan oligosaccharides and modulated gut microbiota

Food Chem X. 2023 Dec 16:21:101075. doi: 10.1016/j.fochx.2023.101075. eCollection 2024 Mar 30.

Authors

Huan Li¹, Wenjiang He², Saiqing Xu^{1

3}, Rongrong Wang⁴, Shuai Ge^{1

3}, Haishan Xu^{1

3}, Yang Shan^{1

3}, Shenghua Ding^{1

3}

Affiliations

¹ DongTing Laboratory, Hunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Hunan Provincial Key Laboratory for Fruits and Vegetables Storage Processing and Quality Safety, Changsha, 410125, China.
² R&D Centre, Infinitus (China) Company Ltd., Guangzhou, 510520, China.
³ Longping Branch, College of Biology, Hunan University, Changsha, 410125, China.
⁴ College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, China.

Abstract

In this study, the effects of grafting chlorogenic acid (CA) on the antioxidant and probiotic activities of curdlan oligosaccharides (CDOS) were investigated. CDOS with degrees of polymerization of 3-6 was first obtained by degradation of curdlan with hydrogen peroxide and then grafted with CA using a free radical-mediated method under an ultrasonication-assisted Fenton system. The thermal stability and antioxidant ability of CDOS were enhanced after grafting with CA. In vitro fermentation, supplementation of CDOS-CA stimulated the proliferation of Prevotella and Faecalibacterium while inhibiting the growth of harmful microbiota. Notably, the concentration of total short-chain fatty acids and the relative abundance of beneficial bacteria markedly increased after fermentation of CDOS-CA, indicating that CA grafting could improve the probiotic activity of CDOS. Overall, the covalent binding of CDOS and CA could enhance the antioxidant and probiotic activities of CDOS, suggesting potential improvements in gastrointestinal and colonic health.

Keywords: Antioxidant activity; Chlorogenic acid; Curdlan oligosaccharides; Gut microbiota.