Genome-Wide Identification and Expression Analysis of the Stearoyl-Acyl Carrier Protein Δ9 Desaturase Gene Family under Abiotic Stress in Barley

Mingyu Ding; Danni Zhou; Yichen Ye; Shuting Wen; Xian Zhang; Quanxiang Tian; Xiaoqin Zhang; Wangshu Mou; Cong Dang; Yunxia Fang; Dawei Xue

doi:10.3390/ijms25010113

Genome-Wide Identification and Expression Analysis of the Stearoyl-Acyl Carrier Protein Δ9 Desaturase Gene Family under Abiotic Stress in Barley

Int J Mol Sci. 2023 Dec 21;25(1):113. doi: 10.3390/ijms25010113.

Authors

Mingyu Ding¹, Danni Zhou¹, Yichen Ye¹, Shuting Wen¹, Xian Zhang^{1

2}, Quanxiang Tian^{1

2}, Xiaoqin Zhang^{1

2}, Wangshu Mou^{1

2}, Cong Dang^{1

2}, Yunxia Fang^{1

2}, Dawei Xue^{1

2}

Affiliations

¹ College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
² Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou 311121, China.

Abstract

Stearoyl-acyl carrier protein (ACP) Δ9 desaturase (SAD) is a critical fatty acid dehydrogenase in plants, playing a prominent role in regulating the synthesis of unsaturated fatty acids (UFAs) and having a significant impact on plant growth and development. In this study, we conducted a comprehensive genomic analysis of the SAD family in barley (Hordeum vulgare L.), identifying 14 HvSADs with the FA_desaturase_2 domain, which were divided into four subgroups based on sequence composition and phylogenetic analysis, with members of the same subgroup possessing similar genes and motif structures. Gene replication analysis suggested that tandem and segmental duplication may be the major reasons for the expansion of the SAD family in barley. The promoters of HvSADs contained various cis-regulatory elements (CREs) related to light, abscisic acid (ABA), and methyl jasmonate (MeJA). In addition, expression analysis indicated that HvSADs exhibit multiple tissue expression patterns in barley as well as different response characteristics under three abiotic stresses: salt, drought, and cold. Briefly, this evolutionary and expression analysis of HvSADs provides insight into the biological functions of barley, supporting a comprehensive analysis of the regulatory mechanisms of oil biosynthesis and metabolism in plants under abiotic stress.

Keywords: SAD; abiotic stress; barley; evolution; unsaturated fatty acids (UFAs).

MeSH terms

Acyl Carrier Protein
Fatty Acid Desaturases
Genomics
Hordeum* / genetics
Phylogeny

Substances

Acyl Carrier Protein
Fatty Acid Desaturases

Grants and funding

This research was funded by Hangzhou Normal University Graduate Research Innovtion Promotion Project (grant number: 2022HSDYJSKY223). And The APC was funded by Hanzhou Normal University.