The complete mitochondrial genome of the Tanichthys flavianalis (Cypriniformes: Tanichthyidae) and its phylogeny

Yuzhou Gong; Tiezhu Yang; Chaoqun Su; Liangjie Zhao; Fan Li

doi:10.1080/23802359.2023.2282794

The complete mitochondrial genome of the Tanichthys flavianalis (Cypriniformes: Tanichthyidae) and its phylogeny

Mitochondrial DNA B Resour. 2023 Dec 1;8(12):1325-1329. doi: 10.1080/23802359.2023.2282794. eCollection 2023.

Authors

Yuzhou Gong^{1

2}, Tiezhu Yang^{3

4}, Chaoqun Su^{3

4}, Liangjie Zhao^{3

4}, Fan Li^{1

5}

Affiliations

¹ Branch of Shanghai Science and Technology Museum, Shanghai Natural History Museum, Shanghai, China.
² CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, China.
³ College of Fisheries, Xinyang Agriculture and Forestry University, Xinyang, China.
⁴ Fishery Biological Engineering Technology Research Center of Henan Province, Xinyang, China.
⁵ Institute of Hydrobiology, The Key Laboratory of Aquatic Biodiverstiy and Conservation of Chinese Academy of Sciences, Chinese Academy of Sciences, Wuhan, China.

Abstract

In the present study, we have sequenced, annotated and characterized the complete mitogenome of Tanichthys flavianalis Li, Liao & Shen, 2022, which is an endemic minnow to Hainan Province, China. The entire mitogenome size is 16,545 bp, containing 37 genes coding for 13 mitochondrial proteins, 22 tRNAs, 2 rRNAs, and one putative control region. It has the typical fish mitochondrial gene arrangement, though with 9 gene overlaps and 17 intergenic spacers scattering throughout the mitogenome, and a high A + T bias (60.8%) comparing to other Cyprinids. ATG is used as the start codon in most mitochondrial protein coding genes (PCGs) except for cox1 which uses GTG, whereas the canonical TAA/TAG are prevailing stop codons except for cox2, cox3, nad4 and cytb. Phylogenetic analyses with other reported species from Tanichyidae were conducted using IQ-TREE and MrBayes, based on the concatenated 13 PCGs. The results clustered T. flavinalis with the newly described T. albiventris Li Bohlen & Liao, 2022, and proved the reciprocally monophyletic relationship between Chinese and Vietnamese Tanichthys.

Keywords: Hainan; endemic minnow; long and accurate PCR; mitogenome; protein coding genes.

Grants and funding

The study was supported by the Opening project of CAS key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences [No. KXYSWS2205], the Research special project (basic research direction) of Shanghai Science & Technology Museum [No. SST M/SOP ZY-03-R2KZ-20220100012 and No. SST M/SOP ZY-03-R2KZ-20230100003], the Innovative Research Team of Ecological fisheries in Reservoirs of the Huai River Basin in Xinyang Agriculture and Forestry University [XNKJTD-016], and the Youth Fund Project of Xinyang Agriculture and Forestry College [No. QN2021020].