Exploiting the beneficial effects of Salvia officinalis L. extracts in human health and assessing their activity as potent functional regulators of food microbiota

Vasiliki Kompoura; Ioanna Karapantzou; Gregoria Mitropoulou; Nikolaos A Parisis; Vasileios K Gkalpinos; Vasiliki A Anagnostou; Antonis D Tsiailanis; Efstathios P Vasdekis; Ioannis K Koutsaliaris; Aikaterini N Tsouka; Lefkothea Karapetsi; Panagiotis Madesis; Stavroula Letsiou; Dimitra Florou; Anna-Irini Koukkou; Alexandra Barbouti; Alexandros D Tselepis; Yiannis Kourkoutas; Andreas G Tzakos

doi:10.1016/j.foodchem.2023.138175

Exploiting the beneficial effects of Salvia officinalis L. extracts in human health and assessing their activity as potent functional regulators of food microbiota

Food Chem. 2024 May 30:441:138175. doi: 10.1016/j.foodchem.2023.138175. Epub 2023 Dec 9.

Authors

Vasiliki Kompoura¹, Ioanna Karapantzou¹, Gregoria Mitropoulou¹, Nikolaos A Parisis², Vasileios K Gkalpinos², Vasiliki A Anagnostou², Antonis D Tsiailanis², Efstathios P Vasdekis³, Ioannis K Koutsaliaris⁴, Aikaterini N Tsouka⁴, Lefkothea Karapetsi⁵, Panagiotis Madesis⁵, Stavroula Letsiou², Dimitra Florou⁶, Anna-Irini Koukkou², Alexandra Barbouti⁷, Alexandros D Tselepis⁴, Yiannis Kourkoutas¹, Andreas G Tzakos⁸

Affiliations

¹ Laboratory of Applied Microbiology and Biotechnology, Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece.
² Department of Chemistry, Section of Organic Chemistry and Biochemistry, University of Ioannina, 45110 Ioannina, Greece.
³ Directorate of Rural Economy, Region of Epirus, Ioannina, Greece.
⁴ Department of Chemistry, Section of Organic Chemistry and Biochemistry, University of Ioannina, 45110 Ioannina, Greece; Atherothrombosis Research Centre/Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110, Ioannina, Greece.
⁵ Laboratory of Molecular Biology of Plants, Department of Agriculture Crop Production and Rural Environment, University of Thessaly, Fytokou St., N. Ionia, 38446 Magnesia, Greece; Institute of Applied Biosciences (INAB), Centre for Research and Technology Hellas (CERTH), 6th Km Charilaou-Thermi Road, 57001 Thessaloniki, Greece.
⁶ Department of Forensic Medicine & Toxicology, Faculty of Medicine, School of Health Sciences, University of Ioannina, 45110 Ioannina, Greece.
⁷ Department of Anatomy-Histology-Embryology, Faculty of Medicine, School of Health Sciences, University of Ioannina, 45110 Ioannina, Greece.
⁸ Department of Chemistry, Section of Organic Chemistry and Biochemistry, University of Ioannina, 45110 Ioannina, Greece; University Research Center of Ioannina, Institute of Materials Science and Computing, Ioannina, Greece. Electronic address: atzakos@uoi.gr.

PMID: 38194793
DOI: 10.1016/j.foodchem.2023.138175

Abstract

Salvia officinalis L. has attracted scientific and industrial interest due to its pharmacological properties. However, its detailed phytochemical profile and its correlation with beneficial effects in the human microbiome and oxidative stress remained elusive. To unveil this, S. officinalis was collected from the region of Epirus and its molecular identity was verified with DNA barcoding. Phytochemical profile for both aqueous and ethanol-based extracts was determined by high-pressure liquid chromatography-tandem mass spectrometry and 103 phytochemicals were determined. The effect of S. officinalis extracts as functional regulators of food microbiota by stimulating the growth of Lacticaseibacillus rhamnosus strains and by suppressing evolution of pathogenic bacteria was verified. Furthermore, we recorded that both extracts exhibited a significant cellular protection against H₂O₂-induced DNA damage. Finally, both extracts exhibited strong inhibitory effect towards LDL oxidation. This study provides a comprehensive characterization of S. officinalis on its phytochemical components as also its potential impact in human microbiome and oxidative stress.

Keywords: Antimicrobial activity; Growth stimulators; Lacticaseibacillus rhamnosus; Plant extracts; Prebiotics, probiotics; Salvia officinalis.

MeSH terms

Antioxidants / chemistry
Humans
Hydrogen Peroxide
Phytochemicals / analysis
Plant Extracts / chemistry
Salvia officinalis* / chemistry

Substances

Hydrogen Peroxide
Plant Extracts
Phytochemicals
Antioxidants