Copper nitroprusside: An innovative approach for targeted cancer therapy via ROS modulation

Kanwal Asif; Muhammad Adeel; Md Mahbubur Rahman; Michele Bartoletti; Simona Kranjc Brezar; Maja Cemazar; Vincenzo Canzonieri; Flavio Rizzolio; Isabella Caligiuri

doi:10.1016/j.biopha.2023.116017

Copper nitroprusside: An innovative approach for targeted cancer therapy via ROS modulation

Biomed Pharmacother. 2024 Feb:171:116017. doi: 10.1016/j.biopha.2023.116017. Epub 2024 Jan 8.

Authors

Kanwal Asif¹, Muhammad Adeel², Md Mahbubur Rahman³, Michele Bartoletti⁴, Simona Kranjc Brezar⁵, Maja Cemazar⁵, Vincenzo Canzonieri⁶, Flavio Rizzolio⁷, Isabella Caligiuri⁸

Affiliations

¹ Pathology Unit, Centro di Riferimento Oncologico di Aviano (C.R.O.) IRCCS, 33081 Aviano, Italy; Department of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, 30172 Venice, Italy.
² Pathology Unit, Centro di Riferimento Oncologico di Aviano (C.R.O.) IRCCS, 33081 Aviano, Italy; Department of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, 30172 Venice, Italy. Electronic address: muhammad.adeel@unive.it.
³ Department of Applied Chemistry, Konkuk University, Chungju 27478, South Korea.
⁴ Department of Medicine (DAME), University of Udine, Udine, Italy; Unit of Medical Oncology and Cancer Prevention, Department of Medical Oncology, Centro di Riferimento Oncologico di Aviano (CRO), IRCCS, 33081 Aviano (PN), Italy.
⁵ Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska cesta 2, SI-1000 Ljubljana, Slovenia.
⁶ Pathology Unit, Centro di Riferimento Oncologico di Aviano (C.R.O.) IRCCS, 33081 Aviano, Italy; Department of Medical, Surgical and Health Sciences, University of Trieste, 34149 Trieste, Italy.
⁷ Pathology Unit, Centro di Riferimento Oncologico di Aviano (C.R.O.) IRCCS, 33081 Aviano, Italy; Department of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, 30172 Venice, Italy. Electronic address: flavio.rizzolio@unive.it.
⁸ Pathology Unit, Centro di Riferimento Oncologico di Aviano (C.R.O.) IRCCS, 33081 Aviano, Italy.

PMID: 38194739
DOI: 10.1016/j.biopha.2023.116017

Abstract

The clinical application of nanomaterials for chemodynamic therapy (CDT), which generate multiple reactive oxygen species (ROS), presents significant challenges. These challenges arise due to insufficient levels of endogenous hydrogen peroxide and catalytic ions necessary to initiate Fenton reactions. As a result, sophisticated additional delivery systems are required. In this study, a novel bimetallic copper (II) pentacyanonitrosylferrate (Cu(II)NP, Cu[Fe(CN) 5 NO]) material was developed to address these limitations. This material functions as a multiple ROS generator at tumoral sites by self-inducing hydrogen peroxide and producing peroxynitrite (ONOO^-) species. The research findings demonstrate that this material exhibits low toxicity towards normal liver organoids, yet shows potent antitumoral effects on High Grade Serous Ovarian Cancer (HGSOC) organoid patients, regardless of platinum resistance. Significantly, this research introduces a promising therapeutic opportunity by proposing a single system capable of replacing the need for H₂O₂, additional catalysts, and NO-based delivery systems. This innovative system exhibits remarkable multiple therapeutic mechanisms, paving the way for potential advancements in clinical treatments.

Keywords: Copper nitroprusside; Peroxynitrite; Self-supplying hydrogen peroxide; Self-therapeutic.

MeSH terms

Copper*
Humans
Hydrogen Peroxide
Neoplasms*
Nitroprusside
Reactive Oxygen Species

Substances

Copper
Hydrogen Peroxide
Nitroprusside
Reactive Oxygen Species