Array-Based Comparative Genomic Hybridization for the Detection of Copy Number Alterations in Single Cells

Giancarlo Feliciello; Zbigniew Tadeusz Czyz; Bernhard M Polzer

doi:10.1007/978-1-0716-3621-3_11

Array-Based Comparative Genomic Hybridization for the Detection of Copy Number Alterations in Single Cells

Methods Mol Biol. 2024:2752:167-187. doi: 10.1007/978-1-0716-3621-3_11.

Authors

Giancarlo Feliciello¹, Zbigniew Tadeusz Czyz², Bernhard M Polzer³

Affiliations

¹ Cellular and Molecular Diagnostics Group, Division of Personalized Cancer Therapy, Fraunhofer Institute of Toxicology and Experimental Medicine ITEM-R, Regensburg, Germany. giancarlo.feliciello@item.fraunhofer.de.
² Experimental Medicine and Therapy Research, University Regensburg, Regensburg, Germany.
³ Cellular and Molecular Diagnostics Group, Division of Personalized Cancer Therapy, Fraunhofer Institute of Toxicology and Experimental Medicine ITEM-R, Regensburg, Germany.

PMID: 38194034
DOI: 10.1007/978-1-0716-3621-3_11

Abstract

Comprehensive genome-wide analyses of single cells represent an important tool for clinical applications, such as pre-implantation diagnostic and prenatal diagnosis, as well as for cancer research purpose. For the latter, studies of tumor heterogeneity, circulating tumor cells (CTCs), and disseminated cancer cells (DCCs) require the analysis of single-cell genomes. Here we describe a reliable and robust array-based comparative genomic hybridization (aCGH) protocol based on Ampli 1™ whole genome amplification that allows the detection of copy number alterations (CNAs) in single cancer cells as small as 100 kb.

Keywords: Array hybridization; CNAs; PCR labeling; Single cells; aCGH.

MeSH terms

Comparative Genomic Hybridization
DNA Copy Number Variations*
Embryo Implantation
Female
Genome-Wide Association Study
Humans
Neoplastic Cells, Circulating*
Pregnancy