Antioxidant activity study and GC-MS profiling of Camellia sinensis Linn

Md Rashed Hasan; Mohammad Majedul Haque; Md Amirul Hoque; Shahin Sultana; Mohammad Mahbubur Rahman; Md Aftab Ali Shaikh; Md Khabir Uddin Sarker

doi:10.1016/j.heliyon.2023.e23514

Antioxidant activity study and GC-MS profiling of Camellia sinensis Linn

Heliyon. 2023 Dec 10;10(1):e23514. doi: 10.1016/j.heliyon.2023.e23514. eCollection 2024 Jan 15.

Authors

Md Rashed Hasan¹, Mohammad Majedul Haque¹, Md Amirul Hoque¹, Shahin Sultana¹, Mohammad Mahbubur Rahman¹, Md Aftab Ali Shaikh^{1

2}, Md Khabir Uddin Sarker¹

Affiliations

¹ Fibre and Polymer Research Division, BCSIR Dhaka Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh.
² Department of Chemistry, University of Dhaka, Dhaka, 1000, Bangladesh.

Abstract

The antioxidant activity of tea leaves extract is a widely researched topic. Tea leaves, particularly those from the Camellia sinensis Linn plant, have garnered attention due to their potential health benefits attributed to their antioxidant properties. Antioxidants are compounds that can neutralize harmful free radicals in the body, which can damage cells and contribute to various health issues, including cancer, cardiovascular diseases, and aging. In this research, the matured tea leaves which has been considered as agricultural waste in Moulovibazar area of Bangladesh have been investigated as a potential source of antioxidant. Methanol was used as solvent for the extraction of antioxidant. DPPH (1,1-diphenyl-2-picryl hydrazyl) scavenging free radical assay method was used to assess the antioxidant activity of the extracts and ascorbic acid was used as positive control. Gas chromatography with mass spectrometric (GC-MS) analysis method was conducted on this extract to investigate the principal components. The half inhibitory concentration (IC₅₀) values of methanol extract and ascorbic acid were found to be 69.51 μg/mL and 10.70 μg/mL, respectively. Caffeine is the main compound (74.47%) among the eight bioactive compounds was identify and quantified by GC-MS.

Keywords: Antioxidant activity; Camellia sinensis Linn; DPPH; GC-MS and caffeine; Matured leaves.