Isolation, purification, and antiosteoporosis activity of donkey bone collagen from discarded bone and its antioxidant peptides

Jie Wang; Huiwen Hou; Yan Li; Wen Tang; Didi Gao; Zengmei Liu; XinQing Gao; Feiyan Zhao; Feng Sun; Haining Tan; Juan Wang

doi:10.1016/j.heliyon.2023.e23531

Isolation, purification, and antiosteoporosis activity of donkey bone collagen from discarded bone and its antioxidant peptides

Heliyon. 2023 Dec 13;10(1):e23531. doi: 10.1016/j.heliyon.2023.e23531. eCollection 2024 Jan 15.

Authors

Jie Wang^{1

2

3

4}, Huiwen Hou^{1

3

4}, Yan Li^{1

3

4}, Wen Tang^{1

3

4}, Didi Gao^{1

3

4}, Zengmei Liu^{1

3

4}, XinQing Gao^{1

3

4}, Feiyan Zhao^{1

3

4}, Feng Sun^{1

3

4}, Haining Tan^{1

3

4

5}, Juan Wang⁶

Affiliations

¹ National Glycoengineering Research Center, Shandong University, Qingdao, 266237, China.
² Qingdao Haier Biotech Co., Ltd., Qingdao, 266237, China.
³ NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, Shandong University, Qingdao, 266237, China.
⁴ Shandong Provincial Technology Innovation Center of Carbohydrate, Shandong University, Qingdao, 266237, China.
⁵ School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, China.
⁶ Jinan Maternity and Child Care Hospital Affiliated to Shandong First Medical University, Jinan, 250001, China.

Abstract

Oxidative stress plays a crucial role in the development of osteoporosis. In this study, it was observed that donkey bone collagen (DC) at a concentration of 500 μg/mL scavenged 17.89 % of 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radicals, indicating its antioxidant properties. Additionally, when an oxidative damage osteoblast model was created using H₂O₂, 100 μg/mL DC demonstrated the ability to enhance cell survival by 27.31 %. Furthermore, 50 μg/mL DC increased the intracellular differentiation marker alkaline phosphatase (ALP) level by 62.65 %. Additionally, the study revealed that DC significantly increased the expression of osteoporosis-related factors in serum and effectively restored the abnormal structure of spongy bone in mice osteoporosis model. Peptides (GGWFL, ANLGPA, and GWFK) isolated from DC through gastrointestinal digestion and subsequent enzymatic purification in vitro demonstrated the ability to safeguard osteoblasts from H₂O₂-induced damage by reducing intracellular reactive oxygen species (ROS). This protection resulted in enhanced cell survival and promoted osteoblast differentiation. This investigation underscores that DC can shield oxidative damage osteoblast model from oxidative stress, ameliorate osteoporosis, and enhance bone density in mice osteoporosis model. These findings suggest various DC applications in the food and medicine industries.

Keywords: Antioxidant; Donkey bone collagen; Osteoporosis; Oxidative stress; Polypeptides.